FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1144664
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACACCCAAGCACCGCTGCTGTCTGCTGATTGA45660.3988943480357555No Hit
CCCAAGCACCGCTGCTGTCTGCTGATTGACAGTGATAGTCAGCCTCGTCT39620.3461277719924799No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC38380.3352948987650524No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35950.31406596171452933No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGC32600.284799731624302No Hit
GTGCTTGGGTGTTCGGCGGCGGGACCAAGTTGACCGTCCTGAGTCAGCCC31900.27868439996365746No Hit
GTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA30880.26977348811528973No Hit
CCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGTGTCTCATAAGT30450.26601692723803666No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCGAAG29820.26051312874345656No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGT29540.2580669960791988No Hit
CACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG28450.24854455106476658No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG27750.24242921940412207No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG26800.23412984072181878No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACAC26750.23369303131748706No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC26470.23124689865322923No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG25500.22277279620919327No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT25250.22058874918753452No Hit
GGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC25130.21954040661713833No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24360.21281354179042933No Hit
GTGATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA24060.21019268536443883No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC23330.20381526806119524No Hit
GACTATCACTGTCAATCAGCAGACAGCAGCGGTGCTTGGGTGTTCGGCGGCGGGACCAAGTTGACCGTCCTGAGT23240.20302901113339808No Hit
CTTATGAGACACACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGA23050.20136913539693743No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGC22800.1991850883752787No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22710.19839883144748155No Hit
GACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACACCCAAGCACCGCTGCTGTCTGCTGATTGACAGTGATAGT22570.19717576511535262No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATG22170.1936812898806986No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG22110.1931571185955005No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21130.18459565427059818No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20500.17909185577601813No Hit
CTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTCGAGTGGATGGGATGGATCAAACCTAACAGTGGTGA20470.17882977013341908No Hit
GTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC20170.17620891370742855No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20170.17620891370742855No Hit
GTATCAACGCAGAGTACGGGGTGGGTCCAGGAGGCAGAACTCTGGGTGTC19300.1686084300720561No Hit
TCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAGGG18910.16520131671826843No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18410.1608332226749509No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18170.1587365375341585No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA18050.1576881949637623No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT17810.15559150982296988No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCCTGTTGGCTTGAAG17750.15506733853777177No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17540.15323273903957843No Hit
GTAATACATGGCCGTGTCGTCAGATCTCAGGCCGCTCAACTCCATATAGACTGTGTTGATGGACGTGTCCCTGGT17400.15200967270744953No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACACC17200.15026243509012252No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT17030.14877728311539457No Hit
GTGTATATAGTTGCCGATGAAGGTGTATCCAGAAGCCTTGCAGGAGACCTTCACTGAGGCCCCAGGCTTCTTCAC16840.1471174073789339No Hit
GGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATTGCTTCGGCAATGCATCTCCAGAGCAGGTGATCCTGGC16800.1467679598554685No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16700.14589434104680502No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT16400.1432734846208145No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16330.14266195145475002No Hit
GGCCAGGCCCCTGTGGTGTTGATATATAAAGACATTCAGAGGCCCTCAGG16260.1420504182886856No Hit
GCTCTGGAGATGCATTGCCGAAGCAATATGCTTATTGGTACCAGCAGAAG16140.14100207571828938No Hit
GTCTATATGGAGTTGAGCGGCCTGAGATCTGACGACACGGCCATGTATTACTGTGCGAGAGATGAGTCTGAGGTG16110.14073999007569032No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT16040.1401284569096259No Hit
TCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATCACTGTCAATCAGCA15810.13811913364969983No Hit
ACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACACCCAAGCACCGCTGC15710.13724551484103634No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15670.13689606731757092No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACACCCAAGCACCG15650.13672134355583823No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15640.13663398167497187No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15620.13645925791323918No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC15330.13392576336811501No Hit
CCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATTGCTTC15220.13296478267858516No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCACGCGACCCCGAGAGCCT15140.13226588763165437No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT14950.1306060118951937No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14910.13025656437172828No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14890.13008184060999559No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCATCGG14690.1283346029926686No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14640.12789779358833683No Hit
ATGTATTACTGTGCGAGAGATGAGTCTGAGGTGCCTACGATTTTGTCCTACTTTGACTACTGGGGCCAGGGAACC14640.12789779358833683No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC14450.12623791785187619No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC14340.12527693716234634No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA14270.12466540399628187No Hit
ACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAATCGCTCAGGGATCCCT14140.12352969954501933No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13890.12134565252336056No Hit
GTGGAGTCCAGGCAGAAGACGAGGCTGACTATCACTGTCAATCAGCAGAC13810.12064675747642976No Hit
CCGCTGCTGTCTGCTGATTGACAGTGATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA13290.11610393967137955No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAACTTCGGCACCCAGAC13290.11610393967137955No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC12940.1130462738410573No Hit
CCACTGATGGTCAACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAATCG12830.11208529315152743No Hit
AGACACACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC12740.11129903622373027No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG12710.11103695058113122No Hit
CCCCTGTGGTGTTGATATATAAAGACATTCAGAGGCCCTCAGGGATCCCT12550.10963916048726964No Hit
CTCCACTGATGGTCAACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCT12540.10955179860640328No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG12320.10762983722734357No Hit
GGACTCCACTGATGGTCAACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGC12220.10675621841868008No Hit
GTCCAGGCAGAAGACGAGGCTGACTATCACTGTCAATCAGCAGACAGCAG12020.10500898080135306No Hit
AGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCT12000.10483425703962036No Hit
GGATCACCTGCTCTGGAGATGCATTGCCGAAGCAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCC11930.10422272387355591No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAGGGCC11760.10273757189882796No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCCT11730.1024754862562289No Hit
ACTTGGTCCCGCCGCCGAACACCCAAGCACCGCTGCTGTCTGCTGATTGACAGTGATAGTCAGCCTCGTCTTCTG11620.10151450556669904No Hit
CAGTCACGTTGACCATCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTAT11610.10142714368583271No Hit
TCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCC11520.10064088675803555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2300.053.8750881
TGGTATC2350.052.6942372
GGTATCA7650.046.7934651
GTATCAA18200.037.06791
TCTGCCG501.8830391E-434.43224328
ATCAACG20850.032.318473
TCAACGC21100.031.9369414
CAACGCA21800.031.388695
AACGCAG22050.031.3433286
CGCAGAG23200.029.7909748
TTCGGGG10050.029.73147270
GCTTAAG605.496156E-428.6834951
AGAGTAC23800.028.46203211
GCAGAGT24100.028.2504149
CCTTTGT8900.027.9776570
CAGAGTA24350.027.96036510
TCTTCAC7500.027.42011869
ACGGGAA658.7951176E-426.46665216
ACGCAGA26200.026.3797937
GAGTACG22250.024.72564312
Produced by FastQC (version 0.11.5)