FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908967
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT42760.2239954907549476No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19700.10319717417849548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG16600.044.69304370
CTCGTAT9150.044.1802339
CTTATAC18750.042.6676561
CGTAGAG18150.042.52987728
TCGTATG12450.041.1686140
CGTATGC13850.040.6078941
ACGAGAC19300.040.04239722
TCTCGTA9150.039.94435538
ACGTAGA19400.039.93018327
GAGACGT19450.039.57798424
AGACGTA19700.039.09213325
GTATCAA98400.038.6532331
GACGTAG20050.038.59816726
TATACAC21850.038.141573
ATCTCCG20650.038.0592410
CGAGACG21150.036.87005623
CCCACGA21700.036.84789319
TTATACA21700.036.8449972
TCCGAGC21250.036.6657913
CACGAGA21000.036.6317821
Produced by FastQC (version 0.11.5)