FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908967
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG155970.8170387439908601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA106150.040.270531
GTGGTAT14300.037.4256251
GGTATCA44500.036.9172821
TATCAAC116800.036.4045522
CAACGCA131550.032.238745
ATCAACG132850.032.1525653
TCAACGC132350.032.1172264
AACGCAG134950.031.5050496
CGCAGAG142400.029.8337968
ACGCAGA144200.029.508347
TGGTATC18500.029.0960672
AGAGTAC145750.029.05441511
CAGAGTA148550.028.50677110
GCAGAGT150050.028.2902189
CGAATAT657.1303966E-427.43569256
GGTTACG659.6695276E-426.0414712
AGTACGG97350.025.9738713
GAGTACG98000.025.87135312
TACGGGT17950.023.95298215
GTACGGG104350.023.09372714
Produced by FastQC (version 0.11.5)