FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831639
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27380.3292293891941095No Hit
GTATTACTGTCAATGTTTTGGTAGCTCACCTTTATTCACTTTCGGCCCTGGGACCAAAGTGGAGATCAAACGAA23910.2875045542597209No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCA21070.25335512163330487No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18950.22786329164457175No Hit
GTGTATTACTGTCAATGTTTTGGTAGCTCACCTTTATTCACTTTCGGCCC18250.21944617796904667No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18130.21800324419609954No Hit
GTCTGGGACAGACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATT18120.2178829997150206No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17970.21607933249883662No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16860.20273219509907545No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA15120.1818096553913417No Hit
CTCCTGCAGGGCCAGTCAGAGTGTAAGCAGCAGCTACTTAGCCTGGTACC14990.1802464771373156No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14770.17760109855357914No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14680.17651889822386876No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14240.17122814105639586No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA14050.1689434959158962No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA13620.16377298322950223No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTAAGCAG12690.15259024648916175No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACTTTGGTCCCAGGGCCGAAAGTG12610.15162829064053032No Hit
GTAATAGACGGCCGTGTCTGCGGCAGTCACAGAGTTCAGCCTCAGGGAGA11820.1421289766352949No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGAC11810.14200873215421594No Hit
CCTTTATTCACTTTCGGCCCTGGGACCAAAGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC11730.14104677630558451No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC11620.1397240870137163No Hit
AAGTAGTACTGTCCGCGGACGTAGTCACCGTCTAATAAAATTCTGGCACA11560.1390026201272427No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11310.13599650810026948No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11070.13311064055437516No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT10890.1309462398949544No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10860.13058550645171765No Hit
GACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAATGTTTTGGTAGCTCA10160.12216839277619256No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT10040.1207254590032454No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACTTTGGTCC10010.12036472556000861No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA9660.11615616872224607No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCGAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGCTGCTTACACTCTGA9520.11447274598714106No Hit
GTACTACTTTGACTACTGGGGCCAGGGAGCCCTGGTCACCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGTCTT9320.11206785636556245No Hit
GAGTGTAAGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTCGCCAGG9270.11146663396016782No Hit
GAATAAAGGTGAGCTACCAAAACATTGACAGTAATACACTGCAAAATCTT9200.11062492259261532No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9180.11038443363045745No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8810.10593538783053706No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8740.10509367646298455No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT8720.10485318750082669No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8710.10473294301974775No Hit
GTCTATTACTGTGCCAGAATTTTATTAGACGGTGACTACGTCCGCGGACA8650.10401147613327419No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8610.10353049820895845No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8600.10341025372787953No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8600.10341025372787953No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC8570.10304952028464273No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8350.1004041417009063No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAATC200.007237798551.96657642
TACGAAA352.2089638E-549.52196544
GTGGTAT2850.049.5125051
TGGTATC3300.043.7826772
GGTATCA9700.041.8683781
GTATCAA24200.040.8171271
TATCAAC28350.034.9467122
ACCGATC501.8921048E-434.402748
ATCAACG29950.033.1946373
TCAACGC30050.032.9696964
CAACGCA30500.032.4832575
AACGCAG30750.032.2191666
ATAGTAC802.8231734E-630.100593
CGCAGAG32950.030.0697738
AGAGTAC33450.029.31351911
ACGCAGA34250.028.92677
GCAGAGT34900.028.1925079
CAGAGTA35600.027.44653510
AGTACCG658.8020123E-426.4620555
GAGTACG24500.026.40042712
Produced by FastQC (version 0.11.5)