FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599431
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG149012.4858574214546794No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC23700.39537494724163413No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC15600.26024680071601236No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10770.17967038741740082No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9820.1638220245532847No Hit
GTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC9770.16298790019201542No Hit
GTATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGG9200.15347888247354574Clontech Universal Primer Mix Long (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8830.14730636220015314No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7520.12545230393489826No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG7520.12545230393489826No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7360.12278310597883661No Hit
GTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGC6820.11377456287712848Clontech Universal Primer Mix Long (95% over 23bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6510.10860299183725901Clontech SMART CDS Primer II A (100% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6500.10843616696500513No Hit
TTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCC6320.10543331926443576Clontech Universal Primer Mix Long (95% over 23bp)
GGTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC6160.10276412130837412No Hit
TAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTT6000.10009492335231243Clontech Universal Primer Mix Long (95% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)