FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences657828
Sequences flagged as poor quality0
Sequence length20-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT22520.3423387268404507No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13780.20947724937217632No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7960.1210042746736229No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC7160.1088430410380829No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTC200.00811675650.480438
TGTCGTA856.91216E-1140.44404238
TATACAC6650.039.473423
GTATCAA46100.038.4921881
TCGTATG6600.037.2420440
CTTATAC6750.036.913981
CGTAGTA6850.036.5949530
GGTATCA20250.036.581421
GACGCGT6800.036.2107226
CCGTCTT6550.036.14703847
ACGCGTA6900.035.7213127
CGTATGC6950.034.96959341
CTCGTAT5400.034.5087339
AGACGCG7300.034.18478425
TCTCCGA7500.034.10233311
TGTCGGT400.002815533433.93217531
CGAGACG7450.033.91815623
TTATACA7450.033.8820572
ATTGTCG400.002855963333.8336429
TATCAAC54150.033.8115582
Produced by FastQC (version 0.11.5)