FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences657828
Sequences flagged as poor quality0
Sequence length20-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54880.8342606273980433No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC8390.12754093775272562No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAT452.1007443E-457.47462570
GCGTAGT352.6372822E-548.0577123
GTGGTAT6350.043.371721
CGATCTA359.17154E-442.63491466
CTGTCGG350.00105524741.43708859
GTATCAA54400.040.1310921
GACGGTT459.791012E-538.4780140
TATCAAC60100.036.355972
GGTATCA23350.035.2407951
CGGGTCC502.1733229E-433.59942218
GTCCCAC400.002960406233.5866361
ATCAACG67350.032.5321433
TCAACGC67250.032.3784834
CAACGCA68100.031.8758135
CGGCTAT553.0645568E-431.68837442
AACGCAG69200.031.3253966
AAGCGAC450.00525744529.86842519
ACGCAGA74100.029.3036867
CGTCGCA500.005936143529.13520861
TACGGCG704.6550813E-528.7579084
Produced by FastQC (version 0.11.5)