FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences959394
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16590.17292165679585236No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC14800.1542640458456067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11840.12341123667648536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCGC401.037326E-650.072219
GTGGTAT10600.048.487491
CTTATAC11950.045.24411
TATACAC12550.043.8673933
TTATACA12800.042.22862
ATTATCG406.0803948E-541.7203118
TACACAT13700.040.6721885
ACACATC14450.038.5611726
GACTCCT13800.037.5720526
CGAGACT14350.037.24500723
AGGGGGG11650.037.1524270
CTCCGAG14700.036.9955312
ATACACA15000.036.9248244
TCCGAGC14750.036.8701213
ATACGGG1001.2732926E-1136.7119521
GTATCAA80700.036.641651
GTTTCGC551.4023342E-436.20093568
CCCACGA14950.035.9492719
AGACTCC14700.035.6951325
ACGAGAC15250.035.03779622
Produced by FastQC (version 0.11.5)