FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1304271
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36770.28191993841770613No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT32390.2483379604392032No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28610.21935625341665954No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC27680.21222583343492263No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT27250.2089289725831518No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT27070.20754889129636403No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG27060.2074722201137647No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG25850.19819500701924678No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24570.18838109564653358No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC24400.1870776855423451No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT23860.18293744168198173No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23410.17948723846501227No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23060.176803747074036No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22860.17527032342204957No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTC22080.16928997117930245No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21790.16706650688392213No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21530.16507305613633977No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG21440.16438301549294584No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20260.15533581594622592No Hit
AGTTTACAGAGAGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCAGAATCAGCAGT19650.1506588738076673No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19490.14943213488607812No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT19460.14920212133828015No Hit
GATCTATGCTGCATCTAGTTTACAGAGAGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA19360.14843540951228695No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC19200.14720867059069778No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG18870.14467852156492017No Hit
GTAATATAAGGCCGTGTCCTCGACTCTCAGGTTGTCCATTCGCAGATCCA18700.1433751114607317No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA18410.1411516471653514No Hit
GTAGTAAGTTGCAAGATCTTCAGGTTGCAGACTGCTGATTCTGAGAGTGAAATCTGTCCCAGATCCACTGCCA18100.1387748405047724No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC17660.13540130847040224No Hit
ACTTACTACTGTCAACAGAGTTCCACTATTCCCGTGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAA17540.13448125427921037No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA17480.13402122718361445No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC17300.13264114589682666No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17290.13256447471422733No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT17210.13195110525343276No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17090.1310310510622409No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG17010.13041768160144632No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16980.13018766805364834No Hit
GCCTTATATTACTGTGCGAAAGATGTAGGTGTAACCAAATATAGGAGCAGTTGGTACTACTTTGACTCCTGGGGC16670.12781086139306938No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16430.12597075301068567No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16150.12382395989790466No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA15970.12244387861111686No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTGACACCTATTTAAATTGGTATCAGCAGAAA15930.12213719388071959No Hit
ATTCTACGCAGCGTCCGTGAAGGGCCGGTTCACCATCTTCAGAGACAATT15870.12167716678512365No Hit
CTGTAAACTAGATGCAGCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTT15680.12022041431573652No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG15430.11830363475075348No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGGCATAGCTGC15330.11753692292476028No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC14680.11255329605580436No Hit
ATATAAGGCCGTGTCCTCGACTCTCAGGTTGTCCATTCGCAGATCCACCG14410.11048317412562267No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC14320.10979313348222876No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14180.10871973692583826No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14120.10825970983024233No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC14090.10802969628244438No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG14090.10802969628244438No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTGACAC13990.10726298445645115No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC13880.10641960144785861No Hit
GCGTAGAATGTGTCACCCGCAGTAGCACTAATACCTGCGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACC13860.10626625908265996No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT13810.10588290316966337No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT13780.10565288962186539No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA13770.10557621843926607No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA13750.10542287607406745No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG13530.10373611005688235No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13480.10335275414388576No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG13430.10296939823088913No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT13400.10273938468309117No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13370.1025093711352932No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13290.10189600167449862No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13170.10097594748330677No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG13110.10051592038771084No Hit
GTATTAGTGCTACTGCGGGTGACACATTCTACGCAGCGTCCGTGAAGGGCCGGTTCACCATCTTCAGAGACAATT13060.10013256447471423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2300.050.9302831
GTATCAA19550.040.532751
CGCTAAT2450.038.022432
CTCGTAT2050.037.12043839
TATGCCG2550.036.69482843
TATCAAC21700.036.3441242
AATCTCG2100.036.1892336
CGTATGC2600.035.96279541
TCGTATG2550.035.29218740
GCTAATC2200.034.5150633
TCGCTAA2700.034.48723231
ATCAACG23100.034.2892233
CTCGCTA2750.033.85499230
ACGAGAC2750.033.81474722
TCAACGC23550.033.487784
CAACGCA23750.033.205785
CTTATAC2600.033.1277281
ATGCCGT2850.032.84108744
AACGCAG24300.032.454216
CTAATCT2350.032.3219134
Produced by FastQC (version 0.11.5)