FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1304271
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT32790.2514048077431761No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32190.24680453678721676No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT29630.2271767140417904No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28560.21897289750366297No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC25420.19489814616747592No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23610.1810206621169987No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG23110.1771871029870326No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23050.17672707589143666No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21960.1683699169881106No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC21570.16537974086673707No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21510.16491971377114112No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT21420.16422967312774722No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21220.1626962494757608No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20770.1592460462587913No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA19910.15265232455524963No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTC19840.15211562627705438No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19500.14950880606867745No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19280.14782204005149235No Hit
GATCTATGCTGCATCTAGTTTACAGAGAGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA18860.14460185038232085No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG18790.1440651521041256No Hit
GTAATATAAGGCCGTGTCCTCGACTCTCAGGTTGTCCATTCGCAGATCCA18700.1433751114607317No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18470.1416116742609473No Hit
AGTTTACAGAGAGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCAGAATCAGCAGT17830.1367047185745907No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC17540.13448125427921037No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT17320.1327944882620253No Hit
GCCTTATATTACTGTGCGAAAGATGTAGGTGTAACCAAATATAGGAGCAGTTGGTACTACTTTGACTCCTGGGGC17230.1321044476186314No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC17170.1316444205230355No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT17110.13118439342743954No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT16960.13003432568844972No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16710.12811754612346665No Hit
ACTTACTACTGTCAACAGAGTTCCACTATTCCCGTGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAA16640.1275808478452714No Hit
GTAGTAAGTTGCAAGATCTTCAGGTTGCAGACTGCTGATTCTGAGAGTGAAATCTGTCCCAGATCCACTGCCA16620.12742750548007278No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16590.1271974919322748No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG16580.12712082074967548No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTGACACCTATTTAAATTGGTATCAGCAGAAA16430.12597075301068567No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT16060.12313391925451075No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC15640.11991372958533925No Hit
CTGTAAACTAGATGCAGCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTT15380.11792027883775688No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA15230.11677021109876705No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15150.11615684163797248No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT15100.11577348572497587No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA14750.1130899943339996No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT14700.11270663842100301No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14530.11140322831681453No Hit
ATATAAGGCCGTGTCCTCGACTCTCAGGTTGTCCATTCGCAGATCCACCG14510.11124988595161589No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14440.11071318767342064No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG14400.11040650294302334No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA14270.10940977756923216No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14150.1084897233780403No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGGCATAGCTGC14100.10810636746504368No Hit
ATTCTACGCAGCGTCCGTGAAGGGCCGGTTCACCATCTTCAGAGACAATT14060.10779968273464641No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG14040.10764634036944776No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA13880.10641960144785861No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA13860.10626625908265996No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTGACAC13810.10588290316966337No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13730.1052695337088688No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13310.10204934403969727No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC13220.10135930339630336No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13180.10105261866590609No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC13130.1006692627529095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22350.042.3219221
GTGGTAT2700.038.217981
CTAATCA450.001699002137.6133569
TATCAAC25000.037.5375752
ATCAACG25900.035.9456673
TCAACGC26300.035.3989684
CAACGCA26950.034.5531355
AACGCAG27450.034.303246
CGCAGAG29150.032.4230888
AGAGTAC29850.031.66274611
GCAGAGT30150.031.2337069
CAGAGTA32600.029.09723510
CGGGATT1103.774221E-828.1412517
ATAGCGG500.007045014828.13361563
AATAGCG500.007105970828.08401762
ACGCAGA34250.027.3944327
AGTACGG26950.027.28759613
GAGTACG27500.027.2448312
CACTCGG1000.00882055126.8651770
AATACTA658.8628946E-426.4322195
Produced by FastQC (version 0.11.5)