FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences920082
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22370.24313050358555No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA20800.2260668070889334No Hit
GTATAATAATTGGCCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT18940.2058512176088653No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16270.1768320649681224No Hit
GTCTGGGACAGAGTTCACTCTCACCATCGGCAGCCTGCAGTCTGAAGATT14880.16172471584054465No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14240.15476881408396206No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14200.15433407022417567No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13260.14411758951919504No Hit
GAGTTCACTCTCACCATCGGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAATTGG13030.14161781232542317No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT12660.13759643162239887No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12420.1349879684636804No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11920.12955367021635028No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTGGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCACACT11520.12520623161848618No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11510.12509754565353956No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTGGCCTGGTA11410.12401068600407354No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAC11170.1214022228453551No Hit
TTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCCGATGGTGAGAGTGAACTCTGTC11140.12107616495051529No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC11120.12085879302062208No Hit
GTGTTAGCACCAACTTGGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCACACTCCTCATCTTTGGTGCATCCA11010.11966324740620944No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT10820.117598214072224No Hit
CTCCCACACTCCTCATCTTTGGTGCATCCACCAGGGCCACTGGTATCCCA10630.11553318073823854No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9910.10770779126208316No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGGCCAATTATTATACTG9680.10520801406831132No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9270.1007518895055006No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)