FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences920082
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21620.2349790562145548No Hit
GTATAATAATTGGCCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT17110.18596168602363702No Hit
TTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCCGATGGTGAGAGTGAACTCTGTC17020.18498351233911758No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA16920.18389665268965158No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14080.15302983864481642No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13480.1465086807480203No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13340.14498707723876786No Hit
GTCTGGGACAGAGTTCACTCTCACCATCGGCAGCCTGCAGTCTGAAGATT12820.13933540706154451No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGGCCAATTATTATACTG11530.12531491758343277No Hit
GAGTTCACTCTCACCATCGGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAATTGG11420.12411937196902016No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11370.12357594214428713No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10920.11868507372169003No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT10820.117598214072224No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10700.11629398249286477No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT10590.11509843687845214No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10580.11498975091350552No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9930.10792516319197637No Hit
GTGTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCCAAGTTGGTGCTA9770.10618618775283073No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9770.10618618775283073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29000.040.0368961
GCGATAA654.904232E-737.65721558
GGTATCA12200.036.603721
TATCAAC33400.034.6408842
ATCAACG33900.034.1225363
TCAACGC34500.033.4296074
CAACGCA35000.032.9574175
AACGCAG35350.032.728226
TGCGATA751.5107216E-632.6127857
CGCAGAG39250.029.5669298
ACGCAGA39500.029.3797937
AGAGTAC39100.029.24129911
GCAGAGT40250.028.7470429
CAGAGTA42000.027.467510
ACTAGCC657.934365E-426.93678960
GTGGTAT7600.026.6673661
AGTACGG32950.024.17210813
TGTTAGA6250.024.15826670
GAGTACG33950.023.56379912
CGAACTA1502.9534931E-822.94947229
Produced by FastQC (version 0.11.5)