FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1084822
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT64010.5900507179979757No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17310.15956534804788253No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC15240.14048387661754647No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12480.11504191471043176No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT11510.10610035563438057TruSeq Adapter, Index 1 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG13200.048.974770
CTTATAC13150.047.4155271
TTATACA14100.044.4347952
TATACAC14700.043.9813773
ATCGTAC554.2566844E-640.78739564
GTGGTAT11900.040.6280331
CCGTCTT15050.039.9414447
CGTATGC15400.039.75463541
TCGTATG15000.039.23644340
CGTCTTC15900.038.37634348
GACTAGG16100.038.2155726
TAGGCAT16050.038.0446429
TACACAT16500.037.971555
CTCGTAT12200.037.85707539
AGACTAG16350.037.60166525
TCGTACC607.4603868E-637.58672365
TGTCGTA1850.037.11463538
GTATCAA90300.037.1093251
GAGACTA16700.036.7830124
TATGCCG16750.036.6052943
Produced by FastQC (version 0.11.5)