FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences778525
Sequences flagged as poor quality0
Sequence length20-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27610.3546450017661604No Hit
GTATTATAGTACTCCTCCACTCACTTTCGGCGGAGGGACCAAGGTAGAGATCAAACGAACTGTGGCTGCACCAT16110.20692977104139237No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14780.18984618348800616No Hit
CTTTTATACAGCTCCAACAATGAGAACTACTTAGCTTGGTACCAGCAGAA14700.18881859927426867No Hit
CCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTCATTGTTGGAGCTGTATAAAAGACT10580.13589801226678655No Hit
CTCCTAAGGTGCTCATTTCCTGGGCATCGACCCGGGAGTCCGGGGTCCCT9010.11573167207218778No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8940.11483253588516747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC6250.042.6556852
GGTATCA21050.042.5605131
GTGGTAT6450.040.821471
GTATCAA54100.039.2511141
TATCAAC62350.034.3162842
TCAACGC65700.032.8266334
ATCAACG65750.032.801673
CAACGCA67400.032.150795
AACGCAG68850.031.523336
CGATTGT850.00606012829.01033470
ACGCAGA74950.028.9102637
CGCAGAG76150.028.3667458
GACCGTT500.007246201427.97038349
TCGTATG756.0388564E-527.681240
AGAGTAC78600.027.35735511
GCAGAGT79250.027.257139
CCTGTCG3050.027.22741140
CAGAGTA80500.026.79658910
CCTCGCA659.125973E-426.29847516
ACGAGAC1058.283678E-726.0580622