Basic Statistics
Measure | Value |
---|---|
Filename | EGAF00005757545 |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 778525 |
Sequences flagged as poor quality | 0 |
Sequence length | 20-76 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 2761 | 0.3546450017661604 | No Hit |
GTATTATAGTACTCCTCCACTCACTTTCGGCGGAGGGACCAAGGTAGAGATCAAACGAACTGTGGCTGCACCAT | 1611 | 0.20692977104139237 | No Hit |
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1478 | 0.18984618348800616 | No Hit |
CTTTTATACAGCTCCAACAATGAGAACTACTTAGCTTGGTACCAGCAGAA | 1470 | 0.18881859927426867 | No Hit |
CCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTCATTGTTGGAGCTGTATAAAAGACT | 1058 | 0.13589801226678655 | No Hit |
CTCCTAAGGTGCTCATTTCCTGGGCATCGACCCGGGAGTCCGGGGTCCCT | 901 | 0.11573167207218778 | No Hit |
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT | 894 | 0.11483253588516747 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGGTATC | 625 | 0.0 | 42.655685 | 2 |
GGTATCA | 2105 | 0.0 | 42.560513 | 1 |
GTGGTAT | 645 | 0.0 | 40.82147 | 1 |
GTATCAA | 5410 | 0.0 | 39.251114 | 1 |
TATCAAC | 6235 | 0.0 | 34.316284 | 2 |
TCAACGC | 6570 | 0.0 | 32.826633 | 4 |
ATCAACG | 6575 | 0.0 | 32.80167 | 3 |
CAACGCA | 6740 | 0.0 | 32.15079 | 5 |
AACGCAG | 6885 | 0.0 | 31.52333 | 6 |
CGATTGT | 85 | 0.006060128 | 29.010334 | 70 |
ACGCAGA | 7495 | 0.0 | 28.910263 | 7 |
CGCAGAG | 7615 | 0.0 | 28.366745 | 8 |
GACCGTT | 50 | 0.0072462014 | 27.970383 | 49 |
TCGTATG | 75 | 6.0388564E-5 | 27.6812 | 40 |
AGAGTAC | 7860 | 0.0 | 27.357355 | 11 |
GCAGAGT | 7925 | 0.0 | 27.25713 | 9 |
CCTGTCG | 305 | 0.0 | 27.227411 | 40 |
CAGAGTA | 8050 | 0.0 | 26.796589 | 10 |
CCTCGCA | 65 | 9.125973E-4 | 26.298475 | 16 |
ACGAGAC | 105 | 8.283678E-7 | 26.05806 | 22 |