FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences778525
Sequences flagged as poor quality0
Sequence length20-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTACTCCTCCACTCACTTTCGGCGGAGGGACCAAGGTAGAGATCAAACGAACTGTGGCTGCACCAT15230.1956263446902797No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14660.1883048071673999No Hit
CTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC13260.17032208342699337No Hit
CCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTCATTGTTGGAGCTGTATAAAAGACT8630.11085064705693458No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8610.1105937510035002No Hit
CTTTTATACAGCTCCAACAATGAGAACTACTTAGCTTGGTACCAGCAGAA8600.11046530297678303No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8330.1069972062554189No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22650.041.882381
GTGGTAT7900.041.7484131
TGGTATC7700.041.4760552
GTATCAA57400.038.977081
CATACCG700.00209585136.03400870
TATCAAC64700.034.290142
CACGTTA400.002765138234.0582322
CGTTTTA400.00277388234.03630419
CTAGACT701.0970198E-633.975074
TCAACGC68350.032.5086984
CAACGCA69300.032.21225
ATCAACG68950.032.1703343
AACGCAG70100.031.9899946
ACGCAGA75650.029.6430767
CGCAGAG76750.029.3067658
AGAGTAC78150.028.65132311
CAGAGTA79400.028.28584910
GCAGAGT79800.028.27189
CGCGTCT1058.7722947E-725.8874310
GAGTACG52350.024.85823812
Produced by FastQC (version 0.11.5)