FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences885921
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACACCCAAGCACCGCTGGTGTCCGCTGATTGA32390.3656082201460401No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC25380.28648152600514043No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23610.26650231792676776No Hit
CCCAAGCACCGCTGGTGTCCGCTGATTGACAGTGATAGTCAGCCTCGTCT22700.25623052168308463No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGC20370.22993020822398386No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATACATTGCCGAAG20260.228688562524198No Hit
CCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGTGTCTCATAAGT20180.22778554746980825No Hit
GTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA20180.22778554746980825No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGT19820.2237219797250545No Hit
GGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC18790.21209566089978676No Hit
GTGCTTGGGTGTTCGGCGGCGGGACCAAGTTGACCGTCCTGAGTCAGCCC17920.20227537218329852No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACAC17910.20216249530149982No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG17690.19967920390192806No Hit
CACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17680.1995663270201293No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17510.19764742002955116No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT16850.19019754583083592No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG16670.18816576195845905No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG16620.1876013775494655No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC16540.18669836249507574No Hit
GACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACACCCAAGCACCGCTGGTGTCCGCTGATTGACAGTGATAGT16330.18432794797730273No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC15790.17823259636017208No Hit
GTGATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATAGTCAACGTGA15400.17383039797002217No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATA15390.17371752108822344No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15290.1725887522702363No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATAC15140.17089559904325555No Hit
GACTATCACTGTCAATCAGCGGACACCAGCGGTGCTTGGGTGTTCGGCGGCGGGACCAAGTTGACCGTCCTGAGT15140.17089559904325555No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14440.16299421731734545No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14130.15949503398158527No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA13870.15656023505481864No Hit
CTTATGAGACACACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGA13690.15452845118244177No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG13550.15294817483725975No Hit
GGCCAGGCCCCTGTGGTGTTGATATATAAAGACATTCACAGGCCCTCAGG12620.1424506248299792No Hit
GTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC12430.1403059640758036No Hit
TCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAGGG12220.13793554955803056No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12170.137371165149037No Hit
GTAATACATGGCCGTGTCGTCAGATCTCAGGCCGCTCAACTCCACATAGACTGTGTTGATGGACGTGTCCCTGGT11800.13319472052248452No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11680.13184019794089993No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11680.13184019794089993No Hit
GGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATACTGCTTCGGCAATGTATCTCCAGAGCAGGTGATCCTGGC11610.1310500597683089No Hit
GTATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGGTGTTGA11560.13048567535931535No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA11110.12540621567837312No Hit
GTATCAACGCAGAGTACGGGGTGGGTCCAGGAGGCAGAACTCTGGGTGTC11010.12427744686038598No Hit
CCGCTGGTGTCCGCTGATTGACAGTGATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATAGTCAACGTGA10960.1237130624513924No Hit
CCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAACAGT10910.12314867804239882No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT10820.12213278610621037No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACAGCTTCATCGG10780.1216812785790155No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10740.12122977105182066No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10680.12055250976102837No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT10630.11998812535203478No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACACC10620.11987524847023606No Hit
GATTGACAGTGATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATAGT10620.11987524847023606No Hit
ACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACACCCAAGCACCGCTGG10600.11964949470663862No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT10420.11761771083426174No Hit
CCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATACTGCTTC10390.11727908018886558No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10360.11694044954346945No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10250.11569880384368358No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10210.11524729631648871No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACACCCAAGCACCG10190.11502154255289129No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCCTGTTGGCTTGAAG10110.11411852749850156No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC9720.10971632910835163No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9710.10960345222655293No Hit
CACCAGCGGTGCTTGGGTGTTCGGCGGCGGGACCAAGTTGACCGTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGT9610.10847468340856577No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9470.10689440706338375No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9440.10655577641798761No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9420.10633002265439018No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9300.1049755000728056No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9270.10463686942740943No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC9270.10463686942740943No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC9000.10158919361884411No Hit
GGATCACCTGCTCTGGAGATACATTGCCGAAGCAGTATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCC8940.10091193232805182No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC8900.10046042480085697No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAT150.002382732268.7992861
GTGGTAT2800.056.51371
TGGTATC3200.049.418782
GGTATCA7350.035.56971
GTATCAA14550.033.8085861
CTAATTG450.00447974830.86339648
AATACGT450.00454890630.76690941
CTATATG450.00470272730.5584722
ATCAACG17000.028.9116733
TCAACGC17150.028.6571854
CAACGCA17300.028.4135235
AACGCAG17700.027.7745426
CTGTTTA1000.007733029327.59766870
CGACGAA500.00781446127.541528
CGCAGAG19300.025.8282438
AGAGTAC19250.025.35956411
CAGAGTA19500.025.2107410
CCCTACC700.001361352224.5559148
GCAGAGT20050.024.5191739
ACGCAGA20450.024.2076917
Produced by FastQC (version 0.11.5)