FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122226
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG107990.9622838893413626No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC18940.16877170908533573No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC11880.10586102977475126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT11250.044.5397031
TACGAGT358.155171E-443.667267
GTATCAA97000.037.916141
GGTATCA45300.035.690751
CGATTGG501.9073246E-434.3570538
TATCAAC107350.034.2019162
CAAATCG450.004531829630.79119940
ATCAACG119450.030.740613
TCAACGC119300.030.6674394
CAACGCA121400.030.2220555
AACGCAG121900.029.991326
GCGATTG704.046621E-529.3572637
CGATATT606.309132E-428.01486828
ACGCAGA131750.027.702157
CGCAGAG133000.027.5922978
TGGTATC18500.027.4240532
TCGTCTA807.687312E-526.71833448
GCAGAGT138400.026.5880349
AGAGTAC139200.026.43522811
CAGAGTA139800.026.41722910
Produced by FastQC (version 0.11.5)