FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1143185
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAGCACCGCTGGTGTCCGCTGATTGACAGTGATAGTCAGCCTCGTCT40190.35156164575287463No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC38730.3387903095299536No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATACATTGCCGAAG36780.3217327029308467No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35950.3144722857630217No Hit
CCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGTGTCTCATAAGT32520.28446839312972094No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG31010.27125968237861764No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGT30980.2709972576617083No Hit
GCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACACCCAAGCACCGCTGGTGTCCGCTGATTGA30860.26994755879407095No Hit
CACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG28940.25315237691187337No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC28890.25271500238369116No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG28550.249740855592052No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGC28390.24834125710186888No Hit
GTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA27850.24361761219750086No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG27420.2398561912551337No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27340.23915639201004213No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC27330.23906891710440567No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATA27140.23740689389731318No Hit
GACTATCACTGTCAATCAGCGGACACCAGCGGTGCTTGGGTGTTCGGCGGCGGGACCAAGTTGACCGTCCTGAGT26550.2322458744647629No Hit
GGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC26500.2318084999365807No Hit
GTGCTTGGGTGTTCGGCGGCGGGACCAAGTTGACCGTCCTGAGTCAGCCC25790.22559778163639305No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT25210.22052423710947922No Hit
GTGATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATAGTCAACGTGA25170.22017433748693344No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATAC24570.21492584314874671No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA23730.20757795107528526No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG23050.20162965749200698No Hit
GGCCAGGCCCCTGTGGTGTTGATATATAAAGACATTCACAGGCCCTCAGG22090.19323206655090822No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21880.19139509353254283No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21810.19078276919308773No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21660.18947064560854104No Hit
CTTATGAGACACACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGA21650.1893831707029046No Hit
GTATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGGTGTTGA21300.186321549005629No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC21200.18544679994926455No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA20710.18116052957307874No Hit
TCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAGGG20080.17564961051798267No Hit
GTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC20060.17547466070670978No Hit
CCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAACAGT20030.17521223598980043No Hit
GTATCAACGCAGAGTACGGGGTGGGTCCAGGAGGCAGAACTCTGGGTGTC18780.16427787278524472No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT18250.1596417027865131No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18180.159029378447058No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT17750.15526795750469086No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17280.1511566369397779No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACAC17230.15071926241159567No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCCTGTTGGCTTGAAG17140.14993198826086768No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC17030.14896976429886677No Hit
CCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATACTGCTTC16740.14643299203540983No Hit
CCCCTGTGGTGTTGATATATAAAGACATTCACAGGCCCTCAGGGATCCCT16660.14573319279031827No Hit
GGAGATACATTGCCGAAGCAGTATGCTTATTGGTACCAGCAGAAGCCAGG16400.1434588452437707No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16250.142146721659224No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16230.14197177184795112No Hit
GTAATACATGGCCGTGTCGTCAGATCTCAGGCCGCTCAACTCCACATAGACTGTGTTGATGGACGTGTCCCTGGT16170.14144692241413245No Hit
GCTCTGGAGATACATTGCCGAAGCAGTATGCTTATTGGTACCAGCAGAAG16080.14065964826340444No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15910.13917257486758486No Hit
GATTGACAGTGATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATAGT15730.13759802656612885No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT15600.13646085279285505No Hit
GGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATACTGCTTCGGCAATGTATCTCCAGAGCAGGTGATCCTGGC15590.1363733778872186No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15560.13611095317030927No Hit
CCGCTGGTGTCCGCTGATTGACAGTGATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATAGTCAACGTGA15070.13182468279412343No Hit
GGATCACCTGCTCTGGAGATACATTGCCGAAGCAGTATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCC15070.13182468279412343No Hit
CACCAGCGGTGCTTGGGTGTTCGGCGGCGGGACCAAGTTGACCGTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGT14990.13112488354903187No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14830.12972528505884875No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC14740.12893801090812074No Hit
GACTATCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATCACTGTCAAT14650.12815073675739275No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14560.1273634626066647No Hit
CCACTGATAGTCAACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAATCG14340.1254390146826629No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14170.12395194128684334No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA14050.122902242419206No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13750.12027799525011264No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACAGCTTCATCGG13730.12010304543883975No Hit
AGCCAGGCCAGGCCCCTGTGGTGTTGATATATAAAGACATTCACAGGCCC13590.11887839675992949No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13510.11817859751483792No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAGGGCC13190.11537940053447167No Hit
AGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCT13070.11432970166683433No Hit
GACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACACCCAAGCACCGCTGGTGTCCGCTGATTGACAGTGATAGT12930.11310505298792409No Hit
GCAGAAGCCAGGCCAGGCCCCTGTGGTGTTGATATATAAAGACATTCACAGGCCCTCAGGGATCCCTGAGCGATT12760.11161797959210451No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC12760.11161797959210451No Hit
GTGGAGTCCAGGCAGAAGACGAGGCTGACTATCACTGTCAATCAGCGGAC12730.11135555487519519No Hit
GTCCAGGCAGAAGACGAGGCTGACTATCACTGTCAATCAGCGGACACCAG12720.11126807996955873No Hit
GTGTTGATATATAAAGACATTCACAGGCCCTCAGGGATCCCTGAGCGATT12560.10986848147937561No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12530.10960605676246626No Hit
CTGTCAATCAGCGGACACCAGCGGTGCTTGGGTGTTCGGCGGCGGGACCA12500.10934363204555693No Hit
GCAGTATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGGTGTTGATATATAAAGACATTCACAGGCC12370.10820645827228313No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG12350.10803150846101024No Hit
GGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAACA12300.10759413393282803No Hit
CTATCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATCACTGTCAATCA12160.10636948525391778No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA12140.10619453544264489No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCCT12130.10610706053700844No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC12050.10540726129191688No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA12000.10496988676373464No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11950.10453251223555243No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC11910.10418261261300665No Hit
CAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAACAGTCACG11850.10365776317918798No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG11820.10339533846227864No Hit
AGACACACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC11770.10295796393409641No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11760.10287048902845997No Hit
CCCTGAGGGCCTGTGAATGTCTTTATATATCAACACCACAGGGGCCTGGC11750.10278301412282352No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGG11680.10217068978336839No Hit
TCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCC11650.10190826506645904No Hit
ATGTATTACTGTGCGAGAGATGAGTCTGAGGAGCCTACGATTTTGTCGTACTTTGACTACTGGGGCCAGGGAACC11630.10173331525518615No Hit
CTGATAGTCAACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAATCGCTC11570.10120846582136749No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11470.10033371676500304No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)