FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914480
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48180.5268567929315021No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39640.4334703875426472No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG37770.4130216079083195No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36850.4029612457352813No Hit
GTATGATAATCTCCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT33970.3714679380631616No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33110.36206368646662584No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30110.3292581576415012No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29920.3271804741492433No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29490.3224783483509754No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGGAACTGTTTAAATTGGTATCAGCAGAAA28030.30651299098941476No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27950.3056381768874114No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26340.28803254308459453No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG26320.2878138395590937No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26050.2848613419648325No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG25660.2805966232175663No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC24370.26649024582276265No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGGAA24080.2633190447030006No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23920.26156941649899396No Hit
GGCTACTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCACTGAAC23470.25664858717522526No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22980.2512903508004549No Hit
TCAGTAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACAGTAT22350.24440118974717873No Hit
TTATCATACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTACTGATGGTGAAAGTAAAATCTGTC22170.24243285801767125No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT21870.23915230513515878No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTACTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA21270.23259119937013387No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21000.22963870177587264No Hit
TCGTAGATCAGGAGCCTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT20920.22876388767386932No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20900.22854518414836847No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20560.22482722421485435No Hit
GATCTACGATGCATCCAATTTGAAAACCGGGGTCCCATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTA20280.2217653748578427No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT19790.21640713848307233No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA19680.21520426909281778No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19510.2133452891260607No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19260.2106114950573003No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTACTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA19190.20984603271804741No Hit
AGTCAGGACATTAGGAACTGTTTAAATTGGTATCAGCAGAAACCAGGGAA18680.20426909281777622No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18380.20098853993526378No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18190.19891085644300585No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17590.19234975067798094No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT17400.19027206718572304No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG17250.1886317907444668No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17000.18589799667570642No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16850.18425772023445017No Hit
ATATTACTGTCAACAGTATGATAATCTCCCGATCACCTTCGGCCAAGGGA16750.18316420260694602No Hit
ACATATTACTGTCAACAGTATGATAATCTCCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA16630.18185198145394102No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16070.17572828273991775No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16020.17518152392616568No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC15620.17080745341614906No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGGAGATTATCATACTGTTGACAGTAATATGTTGCAATATCTTCAGG15410.16851106639839034No Hit
CCCCTAGGCTCCTGATCTACGATGCATCCAATTTGAAAACCGGGGTCCCA15300.1673081970081358No Hit
GTTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAGGCTCCTGATC15090.16501180999037704No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15040.16446505117662497No Hit
GCCTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAACAGTTCCTA14990.1639182923628729No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14760.16140320181961332No Hit
ATCTTCAGGCTGCAGGCTACTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCACTGAACCTTGATGGGAC14410.15757589012334877No Hit
ACCTTGATGGGACCCCGGTTTTCAAATTGGATGCATCGTAGATCAGGAGCCTAGGGGCTTTCCCTGGTTTCTG14210.15538885486834048No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13850.1514521914093255No Hit
ACTGTCAACAGTATGATAATCTCCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTG13520.1478435832385618No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13440.14696876913655849No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13430.14685941737380806No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13400.1465313620855568No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATC13350.14598460327180474No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13250.1448910856443006No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13150.1437975680167964No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13050.14270405038929226No Hit
GTTCCTAATGTCCTGACTCGCCTGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGA12930.1413918292362873No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12860.14062636689703437No Hit
CATTAGGAACTGTTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTA12840.14040766337153354No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12800.1399702563205319No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC12580.13756451754002275No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12290.1343933164202607No Hit
GGCCGAAGGTGATCGGGAGATTATCATACTGTTGACAGTAATATGTTGCA12270.13417461289475988No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12100.1323156329280028No Hit
CCACTGAACCTTGATGGGACCCCGGTTTTCAAATTGGATGCATCGTAGAT12060.13187822587700113No Hit
GTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTACTGATGGTGAAAGTAAAATCTGTCCCAGATCCA12020.13144081882599948No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11760.12859767299448868No Hit
GGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCT11750.12848832123173826No Hit
GTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCT11440.12509841658647536No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCA11270.12323943661971831No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11240.12291138133146706No Hit
GCAGAAACCAGGGAAAGCCCCTAGGCTCCTGATCTACGATGCATCCAATTTGAAAACCGGGGTCCCATCAAGGTT11130.12170851194121249No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11080.12116175312746041No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11070.12105240136471002No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11060.12094304960195959No Hit
CATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTACTTTCACCATCAGTAGCCTGCAGCCTGAAGATATTG11050.12083369783920916No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10950.11974018021170502No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10920.11941212492345378No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10800.11809990377044878No Hit
GTCCCAGATCCACTTCCACTGAACCTTGATGGGACCCCGGTTTTCAAATT10790.11799055200769837No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT10750.1175531449566967No Hit
GTCCTGACTCGCCTGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCAT10670.11667833085469337No Hit
GCCTGAAGATATTGCAACATATTACTGTCAACAGTATGATAATCTCCCGATCACCTTCGGCCAAGGGACACGA10540.11525675793893797No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC10330.11296037092117925No Hit
GTTCAGTGGAAGTGGATCTGGGACAGATTTTACTTTCACCATCAGTAGCC10330.11296037092117925No Hit
GGACATTAGGAACTGTTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAGGCTCCTGATCTACGATGCATC10290.11252296387017759No Hit
GCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGA10210.11164814976817428No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGG10150.11099203919167178No Hit
GATTTTACTTTCACCATCAGTAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACAGTATGATAATCTC10140.11088268742892135No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10000.10935176275041554No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10000.10935176275041554No Hit
GTGGAAGTGGATCTGGGACAGATTTTACTTTCACCATCAGTAGCCTGCAGCCTGAAGATATTGCAACATATTA9970.10902370746216428No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9780.10694602396990639No Hit
GAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGGAACTGTTTAAATTGGTATCAGCAGAAACCAGGGAAAG9760.10672732044440557No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9740.10650861691890473No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC9560.10454028518939724No Hit
GATCAGGAGCCTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAC9540.10432158166389642No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9540.10432158166389642No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT9500.10388417461289477No Hit
TCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTC9420.10300936051089143No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9380.10257195345988977No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9340.10213454640888811No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG9280.10147843583238561No Hit
GTATCAACGCAGAGTACGGGAGTCAGTCCCAACCAGGACACAGCATGGAC9220.10082232525588312No Hit
CTACTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCACTGAACCT9190.10049426996763189No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAACT200.007369965351.7297137
GTGGTAT2600.048.9158251
GTATCAA19150.040.2068671
TCGTATG1250.035.91915540
CTTATAC1151.8189894E-1235.867771
TATCAAC21650.035.7152
CGTATGC1300.034.57185441
GTATAGC400.00264332534.3732831
ATCAACG23000.033.9175153
TACGGAT2950.033.6224670
TCAACGC23250.033.552814
CAACGCA24200.032.2356535
CTCGTAT1101.0477379E-931.39268739
AACGCAG24900.031.3294326
CGCAGAG26750.029.413238
AGAGTAC26450.029.22724311
TGCCGTC1551.8189894E-1229.06931745
ATGCCGT1551.8189894E-1229.03888344
AGACGGA1551.8189894E-1228.83393725
CAGAGTA27050.028.83298510
Produced by FastQC (version 0.11.5)