FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914480
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43650.4773204444055638No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG35960.3932289388504943No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33580.3672032193158954No Hit
GTATGATAATCTCCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT32700.3575802641938588No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31120.34030268567929317No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29380.32127547896072084No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29310.32051001662146794No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27770.30366984515790396No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27080.29612457352812527No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG26190.2863922666433383No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGGAACTGTTTAAATTGGTATCAGCAGAAA26000.2843145831510804No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24960.2729419998250372No Hit
TTATCATACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTACTGATGGTGAAAGTAAAATCTGTC23640.25850756714198236No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23320.255008310733969No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22210.2428702650686729No Hit
GGCTACTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCACTGAAC22140.24210480272942003No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGGAA21760.23794943574490418No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTACTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA21050.23018546058962472No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20870.22821712886011725No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20580.22504592774035517No Hit
TCAGTAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACAGTAT20570.22493657597760477No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC20440.22351500306184935No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20330.22231213367159477No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20310.22209343014609395No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19360.21170501268480446No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18970.20744029393753827No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTACTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA18920.20689353512378617No Hit
GATCTACGATGCATCCAATTTGAAAACCGGGGTCCCATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTA18470.20197270580001747No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18270.19978567054500918No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT18070.19759863529000085No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18040.19727058000174963No Hit
AGTCAGGACATTAGGAACTGTTTAAATTGGTATCAGCAGAAACCAGGGAA17880.19552095179774298No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA17700.1935526200682355No Hit
TCGTAGATCAGGAGCCTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT17660.19311521301723383No Hit
ATATTACTGTCAACAGTATGATAATCTCCCGATCACCTTCGGCCAAGGGA17500.19136558481322719No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17410.19038141894847346No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17330.1895066048464701No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG16800.1837109614206981No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16700.18261744379319395No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT16570.18119587087743855No Hit
ACATATTACTGTCAACAGTATGATAATCTCCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA16480.1802117050126848No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC16380.17911818738518065No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGGAGATTATCATACTGTTGACAGTAATATGTTGCAATATCTTCAGG16320.17846207680867815No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT16210.1772592074184236No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16150.1766030968419211No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15890.17375995101041028No Hit
CCCCTAGGCTCCTGATCTACGATGCATCCAATTTGAAAACCGGGGTCCCA14680.16052838771761002No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14580.15943487009010585No Hit
GTTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAGGCTCCTGATC14320.15659172425859505No Hit
ACCTTGATGGGACCCCGGTTTTCAAATTGGATGCATCGTAGATCAGGAGCCTAGGGGCTTTCCCTGGTTTCTG14220.1554982066310909No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14190.15517015134283965No Hit
GGCCGAAGGTGATCGGGAGATTATCATACTGTTGACAGTAATATGTTGCA13910.152108301985828No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13680.14959321144256846No Hit
GCCTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAACAGTTCCTA13560.14828099028956346No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13260.14500043740705099No Hit
ATCTTCAGGCTGCAGGCTACTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCACTGAACCTTGATGGGAC13020.14237599510104104No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12930.1413918292362873No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12930.1413918292362873No Hit
GTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTACTGATGGTGAAAGTAAAATCTGTCCCAGATCCA12710.13898609045577814No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12640.13822062811652522No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12600.13778322106552357No Hit
ACTGTCAACAGTATGATAATCTCCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTG12550.1372364622517715No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12340.13494007523401277No Hit
CATTAGGAACTGTTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTA12320.13472137170851195No Hit
GTTCCTAATGTCCTGACTCGCCTGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGA12200.13340915055550695No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12080.13209692940250198No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATC11840.12947248709649198No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11720.128160265943487No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC11700.12794156241798618No Hit
GCCTGAAGATATTGCAACATATTACTGTCAACAGTATGATAATCTCCCGATCACCTTCGGCCAAGGGACACGA11310.12367684367071997No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11300.12356749190796956No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11280.12334878838246871No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT11210.12258332604321583No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11120.12159916017846209No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11060.12094304960195959No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11030.12061499431370833No Hit
GATTTTACTTTCACCATCAGTAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACAGTATGATAATCTC10960.11984953197445543No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGGGAGATT10880.11897471787245209No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10880.11897471787245209No Hit
GTGGAAGTGGATCTGGGACAGATTTTACTTTCACCATCAGTAGCCTGCAGCCTGAAGATATTGCAACATATTA10660.11656897909194297No Hit
GGACATTAGGAACTGTTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAGGCTCCTGATCTACGATGCATC10650.11645962732919254No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT10610.1160222202781909No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10550.1153661097016884No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10550.1153661097016884No Hit
CATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTACTTTCACCATCAGTAGCCTGCAGCCTGAAGATATTG10270.11230426034467675No Hit
GTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCT10190.11142944624267344No Hit
GTTCAGTGGAAGTGGATCTGGGACAGATTTTACTTTCACCATCAGTAGCC10130.11077333566617094No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10120.11066398390342051No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10120.11066398390342051No Hit
GTCCTGACTCGCCTGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCAT10080.11022657685241885No Hit
GCAGAAACCAGGGAAAGCCCCTAGGCTCCTGATCTACGATGCATCCAATTTGAAAACCGGGGTCCCATCAAGGTT10040.1097891698014172No Hit
CCACTGAACCTTGATGGGACCCCGGTTTTCAAATTGGATGCATCGTAGAT9850.1077114863091593No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9830.10749278278365847No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9790.10705537573265682No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9720.10628991339340389No Hit
GTCCCAGATCCACTTCCACTGAACCTTGATGGGACCCCGGTTTTCAAATT9510.10399352637564517No Hit
GGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCT9410.10290000874814101No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG9380.10257195345988977No Hit
GGTTTCTGCTGATACCAATTTAAACAGTTCCTAATGTCCTGACTCGCCTGGCAAGTGATGGTGACT9380.10257195345988977No Hit
GAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGGAACTGTTTAAATTGGTATCAGCAGAAACCAGGGAAAG9340.10213454640888811No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCA9310.10180649112063686No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9210.1007129734931327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2200.049.9034581
GTATCAA19950.040.929591
TAGCGTG350.001378333939.2485326
TACGGAT3700.039.09043570
GCTTATA557.680299E-637.4275931
TATCAAC22300.036.4366042
ATCAACG23200.035.159353
TCAACGC23300.035.010374
CAACGCA23350.034.9411285
AACGCAG23750.034.212056
CGCAGAG25700.031.74968
CAGAGTA25450.031.65734510
AGAGTAC25550.031.39925611
ACGCAGA26950.030.1497697
GCAGAGT27100.029.8563779
GAGTACT8950.028.34673912
CTTACCG500.007428026327.82927350
TGTTAGA11550.027.4301970
GCTTGTC1404.984031E-1026.9567781
AGTACTT9200.026.45703313
Produced by FastQC (version 0.11.5)