FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468567
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18710.39930255438389817No Hit
GTTTGATACTCTGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT17950.3830828888931572No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15950.34039955865436533No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG14190.3028382280442285No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13490.2878990624606513No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12960.27658797994737144No Hit
GTATCAAACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAGTGTGTC12680.27061231371394057No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12470.2661305640388674No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12380.26420981417812184No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12140.2590878145494668No Hit
GCTATACACTGGGTCCGCCAGGCTTCCGGGAAAGGGCTGGAGTGGCTTGGCCGTATTAGAAGCAGGGCTGACAGT11280.24073398254678627No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA10390.22173990059052387No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10050.21448373444992924No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9990.21320323454276552No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9840.2100019847748561No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9590.2046665684950071No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9180.19591648579605478No Hit
GTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG8660.18481881993396887No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8560.1826846534220293No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8420.17969682030531386No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8330.17777607044456822No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8300.17713582049098633No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7770.1658247379777065No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7730.16497107137293066No Hit
GAGTATCAAACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGG7730.16497107137293066No Hit
GGCCAAAACTGCACAGAGTATCAAACTGTTGACAGTAATATGTTGCAATA7710.16454423807054275No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC7660.16347715481457295No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7620.1626234882097971No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT7500.16006248839546958No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG7490.15984907174427565No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT6860.1464038227190562No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC6790.14490990616069846No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTGAGGACATTAGGAA6780.14469648950950453No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6760.1442696562071166No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6680.1425623229975649No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT6620.14128182309040116No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAACTG6610.14106840643920718No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6440.13744032336890988No Hit
CACTTTACTTTCACCATCAGCAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACAGTTTGATACT6430.13722690671771592No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTGAGGACATTAGGAACTATTTAAATTGGTATCAGCAGAAA6380.13615982346174615No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGGCAT6380.13615982346174615No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6370.13594640681055217No Hit
TTGATACTCTGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA6360.13573299015935822No Hit
GAATACAGGAGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGT6330.13509274020577633No Hit
CCCCTAAACTCCTGATCTACGATGCATCCACTTTGAAAGCAGGGGTCCCC6220.13274515704264278No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC6200.13231832374025487No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAGTGTGTCCCAGATCCACTTCCA6200.13231832374025487No Hit
ACCTTGAGGGGACCCCTGCTTTCAAAGTGGATGCATCGTAGATCAGGAGTTTAGGGGCTTCCCCTGGTTTCTG6170.13167807378667298No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6130.13082440718189714No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC6100.13018415722831528No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6070.1295439072747334No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAGTGTGTCCCAGATCCACTTCCA6040.1289036573211515No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA6020.1284768240187636No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG6010.12826340736756964No Hit
ACATATTACTGTCAACAGTTTGATACTCTGTGCAGTTTTGGCCAGGGGAC5980.12762315741398778No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT5940.1267694908092119No Hit
ATATTACTGTCAACAGTTTGATACTCTGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG5940.1267694908092119No Hit
CCTCAAGGTTCAGTGGAAGTGGATCTGGGACACACTTTACTTTCACCATCAGCAGCCTGCAGCCTGAAGATATTG5860.12506215759966025No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA5770.1231414077389146No Hit
GTTTAGGGGCTTCCCCTGGTTTCTGCTGATACCAATTTAAATAGTTCCTA5750.1227145744365267No Hit
AAACTGCACAGAGTATCAAACTGTTGACAGTAATATGTTGCAATATCTTC5690.12143407452936293No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC5600.11951332466861729No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT5560.11865965806384146No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA5340.11396449173757435No Hit
GTTCAGTGGAAGTGGATCTGGGACACACTTTACTTTCACCATCAGCAGCC5280.11268399183041058No Hit
GCAGAAACCAGGGGAAGCCCCTAAACTCCTGATCTACGATGCATCCACTTTGAAAGCAGGGGTCCCCTCAAGGTT5270.11247057517921664No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC5260.11225715852802268No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG5260.11225715852802268No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG5220.11140349192324685No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA5170.11033640866727704No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA5120.10926932541130724No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA5070.10820224215533744No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC5060.10798882550414347No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT5060.10798882550414347No Hit
GTGTATAGCAGAGCCACTGAAGCTGAACCCAGAGGCTGCACAGGAGAGTC5000.10670832559697974No Hit
CCACTGAACCTTGAGGGGACCCCTGCTTTCAAAGTGGATGCATCGTAGAT4950.10564124234100994No Hit
CTCCTGTGCAGCCTCTGGGTTCAGCTTCAGTGGCTCTGCTATACACTGGGTCCGCCAGGCTTCCGGGAAAGGG4940.10542782568981597No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT4900.10457415908504014No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC4810.1026534092242945No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC4810.1026534092242945No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA4750.10137290931713074No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG4710.1005192427123549No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGCG150.002378292668.8265421
GTATTGG200.007423631451.6309241
CGACAAG200.007429901551.619914
ATGTATG200.007429901551.619911
GTGGTAT1300.050.3070531
ACAGGTA405.0658604E-543.025771
CTAGATC350.001351646739.4009533
TATCAAC9800.034.4059262
GTATTAT2301.8189894E-1233.8022770
ATCAACG10250.033.2310873
TCAACGC10400.032.7517934
CAACGCA10750.032.3255655
AACGCAG10750.032.3255656
ACGAGAC751.6966806E-632.1259122
GGGAACG1651.6076228E-731.4122170
AGAGTAC11100.031.31297311
CGCAGAG11250.031.1947048
TAGCACT450.004682047330.5830417
GCCGTCT703.6849262E-529.75756346
ATGCCGT703.7120997E-529.72555244
Produced by FastQC (version 0.11.5)