FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1162935
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43430.373451654649658No Hit
GTTTGATACTCTGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT40720.35014854656537125No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36010.30964757273622345No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG34210.2941694935658485No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31640.272070236083702No Hit
GTATCAAACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAGTGTGTC30320.26071964469209374No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29530.25392648772287363No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28230.24274787498871392No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27610.23741653660780698No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27570.23707257929290976No Hit
GCTATACACTGGGTCCGCCAGGCTTCCGGGAAAGGGCTGGAGTGGCTTGGCCGTATTAGAAGCAGGGCTGACAGT25960.22322829736829658No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23730.20405267706277652No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA23090.19854936002442097No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22040.18952048050836892No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21880.18814465124878002No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20740.1783418677742092No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20360.17507427328268563No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20080.17266657207840508No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19660.16905502027198424No Hit
GAGTATCAAACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGG19390.166733308396428No Hit
GTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG19360.16647534041025508No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC19340.16630336175280647No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19110.16432560719214745No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18380.15804838619527314No Hit
GGCCAAAACTGCACAGAGTATCAAACTGTTGACAGTAATATGTTGCAATA18050.1552107383473711No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17800.15306100512926346No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17550.15091127191115583No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTGAGGACATTAGGAA17390.14953544265156693No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTGAGGACATTAGGAACTATTTAAATTGGTATCAGCAGAAA17180.14772966674835653No Hit
CCCCTAAACTCCTGATCTACGATGCATCCACTTTGAAAGCAGGGGTCCCC16790.1443760829281086No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16540.14222634971000098No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16340.14050656313551488No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT16190.1392167232046503No Hit
TTGATACTCTGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA15600.1341433528099163No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15410.13250955556415447No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC15400.1324235662354302No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15290.13147768361946283No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15200.13070377966094407No Hit
ACCTTGAGGGGACCCCTGCTTTCAAAGTGGATGCATCGTAGATCAGGAGTTTAGGGGCTTCCCCTGGTTTCTG15140.13018784368859826No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15130.13010185435987393No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAACTG15130.13010185435987393No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14790.12717821718324754No Hit
ATATTACTGTCAACAGTTTGATACTCTGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG14660.12606035590983158No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGGCAT14650.12597436658110728No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14550.12511447329386424No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAGTGTGTCCCAGATCCACTTCCA14550.12511447329386424No Hit
GTTTAGGGGCTTCCCCTGGTTTCTGCTGATACCAATTTAAATAGTTCCTA14510.12477051597896702No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14360.12348067604810242No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14220.12227682544596215No Hit
ACATATTACTGTCAACAGTTTGATACTCTGTGCAGTTTTGGCCAGGGGAC14160.12176088947361632No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14070.12098698551509758No Hit
CCTCAAGGTTCAGTGGAAGTGGATCTGGGACACACTTTACTTTCACCATCAGCAGCCTGCAGCCTGAAGATATTG14010.12047104954275174No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT13930.1197831349129573No Hit
CACTTTACTTTCACCATCAGCAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACAGTTTGATACT13840.11900923095443856No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13770.1184073056533684No Hit
GAATACAGGAGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGT13740.1181493376671955No Hit
AAACTGCACAGAGTATCAAACTGTTGACAGTAATATGTTGCAATATCTTC13590.11685949773633092No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAGTGTGTCCCAGATCCACTTCCA13080.11247404197139135No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12890.11084024472562955No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12600.10834655419262469No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12490.10740067157665734No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11980.10301521581171777No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11710.10069350393616151No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11680.1004355359499886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG2550.044.77018740
GTGGTAT3250.044.4305651
CGCTAAT2750.042.5988132
CTTATAC3300.041.6736871
AATCTCG2500.041.42449636
CTAATCT2550.039.21615634
TCGCTAA3100.038.88897331
GCTAATC2700.038.29975533
ATGCCGT3000.038.1537744
ACGAGAC3250.038.07187322
GCCGTCT3250.037.392346
CTCGTAT2700.035.8516139
ATCTCGT2800.035.7718337
TTATACA3850.035.6988032
CTCGCTA3400.035.44995530
TATCAAC23450.035.1659852
TATGCCG3300.034.6551643
ACTCGCT3550.033.9403729
TACACAT3900.033.4776275
CACGAGA3700.033.43719521
Produced by FastQC (version 0.11.5)