FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483562
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT29490.19877834563031405No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACGC150.002547226167.65288511
CGTATGC6450.048.92299741
TCGTATG6650.045.24234440
CTTATAC7350.044.6642841
TATGCCG7000.044.3426543
CTCGTAT6300.043.79115339
TCTCGTA5950.043.35592738
TGGTATC14550.043.2493322
GGTATCA41550.041.6222721
GTGGTAT15050.041.3768351
GTATCAA105600.040.413561
GTATGCC7850.040.3065142
TCCGAGC8100.040.09059513
ACGAGAC8150.038.61902622
TGCCGTC8100.038.50951445
ATGCCGT8000.038.46814744
TCTCCGA8550.038.37619411
CTCCGAG8650.037.93253712
AGGGGGG9350.037.44589670
CCCACGA8600.037.3689419
Produced by FastQC (version 0.11.5)