FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483562
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64530.4349666545786425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT15450.041.6011241
GGTATCA42500.040.9123231
GTATCAA108250.040.718941
TGGTATC15450.040.2656672
TATCAAC119400.036.8115962
ATCAACG130350.033.7349473
TCAACGC130650.033.4977464
CAACGCA131900.033.160265
AACGCAG132800.032.9654276
ACGCAGA141200.030.9564277
CGCAGAG143150.030.5347378
TACGGTT450.005105722730.05032315
CCGCGTA604.2696268E-429.94839960
AGAGTAC148900.029.42370211
CAGAGTA151350.028.90272910
GCAGAGT151900.028.7535679
CGCGTAG656.713765E-427.71919361
TCTGTCG4150.027.6960498
GAGTACG110850.026.89799312
AGTACGG109900.026.8835213
Produced by FastQC (version 0.11.5)