FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1973978
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG85480.43303420808134635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT13250.041.5403181
GTATCAA98950.039.04051
GGTATCA46250.034.1595341
TATCAAC114050.033.668112
ATCAACG120300.031.7915423
AACGCAG123600.031.1412416
TCAACGC123200.030.9338054
CAACGCA124050.030.7538785
ACGCAGA132850.028.8977747
CGCAGAG137450.027.9306628
AGAGTAC138400.027.8615711
CAGAGTA138800.027.85464510
TGGTATC20250.027.8313622
GCAGAGT141300.027.1456099
ATTAGCG1307.0324495E-726.55488669
GAGTACG89300.024.5546312
AGTACGG89050.024.3561313
GAGTACT69750.023.7480512
AGTACTT69150.023.51173613
TAACGGC2550.022.91091236
Produced by FastQC (version 0.11.5)