FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684990
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90941.3276106220528767No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC21230.30993153184718025No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC10370.15138907137330473No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7800.11387027547847413No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7630.11138848742317407No Hit
GTATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGG6920.10102337260397962No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTAGT200.008745288549.5369031
GTTACGT200.00878579149.4790236
GTGGTAT12800.047.4728661
CGGGTTA350.001671443337.73688517
TAGATTG350.00167986437.6983034
CTAGCCG400.001744456637.409161
GTATCAA81450.035.477631
TGTGACC400.00249962834.76581641
TCTAGTG800.00255297734.611370
TGGTATC18950.033.6050722
GGTATCA41500.032.467561
CCCGTCC852.0156458E-631.4269865
TATCAAC92950.031.238492
GTATCAG554.2061575E-430.0223671
TCAACGC99700.029.1150364
ATCAACG99850.029.1043383
CAACGCA103450.028.2190635
AACGCAG103900.028.192096
TGACTCC550.00873776326.91680758
ATGTGTT500.00962461726.3888118
Produced by FastQC (version 0.11.5)