FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences907459
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT44660.49214344670117327No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC13780.15185259058535977No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12480.1375268744924013No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9210.10149218862780576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG9000.053.86952670
GTGGTAT7450.045.727841
CTCGTAT7700.045.660739
TCGTATG8750.045.46039240
CTTATAC9150.043.8023031
ATACGGG1000.043.4190331
CGTATGC9400.042.8389541
TATACAC9600.042.4191553
ACGAGAC9400.042.28203622
TGCGATA406.077167E-541.7237635
TTGCGTG651.524677E-841.077339
GTATGCC9750.040.7689742
TTATACA10600.039.6791232
TACACAT10500.038.783235
TCTCGTA8350.038.21486338
GTATCAA69100.038.1843871
ATCGTAT1009.094947E-1237.9162939
CACGAGA10700.036.5146421
AGACCTC10750.036.38693225
CTTGAAA11500.036.1573357
Produced by FastQC (version 0.11.5)