FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences851675
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26650.31291278950303814No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC21670.2544397804326768No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17960.2108785628320662No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT17800.20899991193823936No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10730.12598702556726452No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC10670.12528253148207943No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTTA150.00289415465.5059611
TATATGC253.3177115E-452.4047665
AACGGAC556.12545E-541.66506269
TCGTATG4400.039.4180640
CTTATAC4400.038.717161
TTGTATC2200.038.7148917
TAATCTC3250.038.49733735
GTTCAGT400.001537818238.38271764
AGGGGGG5650.038.3772270
GTGGTAT10550.038.194911
TATACAC4800.037.5294573
CACTGCT2650.037.07884211
GTATCAA101650.036.902091
TAGTGAC400.00209012836.0579247
TACACAT4950.035.0688485
TATGCCG4950.034.91504743
TCTCGTA3750.034.7015538
GTAATCT3700.034.56988534
GGTATCA47400.034.5576741
CTCGTAT3900.034.45648239
Produced by FastQC (version 0.11.5)