FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1354323
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA44670.3298326913151442No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT37890.27977077846274484No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36730.2712056134319509No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAAGACT33410.2466915204127819No Hit
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAGTTCAGTTTCAGGGAGA33090.24432871626635594No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29690.2192239222105805No Hit
GTATAAAAGACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA26190.19338075185904693No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24580.1814928934973415No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24300.1794254398692188No Hit
GTATATTACTGTGCGAAACAGGAACTGGGGATTAATTGGTTCTTCGATCTCTGGGGCCGTGGCACCCTGGTCA24070.17772717438897517No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24030.1774318238706719No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22290.16458407632448094No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22210.16399337528787444No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC21880.16155673351187272No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21740.1605230066978114No Hit
ATATTATGGTATTCCGGGGTTCGGCCCTGGGACCAAAGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT21030.15528053499792885No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20480.1512194653712593No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACTTTGGTCCCAGGGCCGAACCCC19550.14435256582070896No Hit
CCATAATATTGCTGACAGTGATAAACTGCCACATCTTCAGCCTGGAGGCTGCTGATGGTGAGAGTGAAATCTGTC19460.14368802715452664No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18740.13837171782506832No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC18580.13719031575185536No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17780.13128330538579053No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATA17740.1309879548674873No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17730.1309141172379115No Hit
GTTTATCACTGTCAGCAATATTATGGTATTCCGGGGTTCGGCCCTGGGAC17500.12921585175766787No Hit
GTCTCTGGTGGCTCCATCAGCAGGAGTACTTACTACTGGGGCTGGAGCCG17350.1281082873140307No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC16770.12382570479863371No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16760.1237518671690579No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16710.12338267902117885No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16500.12183208880008684No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16440.12138906302263197No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAA16420.12124138776348034No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA16020.11828788258044795No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16000.11814020732129632No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14920.11016574332710884No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14670.10831980258771355No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14660.10824596495813776No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14560.10750758866237965No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14290.10551397266383278No Hit
GTGATAAACTGCCACATCTTCAGCCTGGAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA14210.10492327162722631No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA14190.10477559636807468No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14100.10411105770189238No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTG13970.10315116851740684No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACTTTGGTCC13940.10292965562867942No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13890.10256046748080037No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13740.10145290303716321No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13640.1007145267414051No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG13580.10027150096395025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT10450.046.72641470
GTGGTAT3700.040.910931
GTATCAA24850.039.455441
GGTATCA10950.039.2721181
TGGTATC4100.037.73642
TCGTATG2250.036.90995440
GTCGTAT501.8360374E-434.58123439
TTTAACG501.8646705E-434.49029534
TATCAAC28800.033.9045262
ATCAACG29100.033.436843
TCAACGC29400.032.9787034
CGTATGC2550.032.58455741
CAACGCA29950.032.3730855
AACGCAG30400.032.0069776
CTCGTAT1950.031.9211439
TATACAC2950.031.4683173
ACGAGAC2750.031.27257522
CACGAGA2850.030.17083421
CTACGGA10650.030.06201469
ATCTCCG3050.029.31253810
Produced by FastQC (version 0.11.5)