FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1354323
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33430.24683919567193352No Hit
CTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA33130.2446240667846592No Hit
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAGTTCAGTTTCAGGGAGA31830.23502517493980388No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT31080.2294873527216181No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAAGACT29350.21671344280500293No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25180.18592315127189007No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24390.1800899785354011No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24030.1774318238706719No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23060.1702695738018183No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22960.16953119750606022No Hit
GTATATTACTGTGCGAAACAGGAACTGGGGATTAATTGGTTCTTCGATCTCTGGGGCCGTGGCACCCTGGTCA22650.16724223098921012No Hit
CCATAATATTGCTGACAGTGATAAACTGCCACATCTTCAGCCTGGAGGCTGCTGATGGTGAGAGTGAAATCTGTC22050.1628119732146615No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21980.16229510980763082No Hit
GTATAAAAGACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA19980.14752758389246878No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACTTTGGTCCCAGGGCCGAACCCC19950.14730607100374135No Hit
ATATTATGGTATTCCGGGGTTCGGCCCTGGGACCAAAGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT19770.14597699367137676No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19240.14206359930385884No Hit
GTGATAAACTGCCACATCTTCAGCCTGGAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA19110.1411037101193733No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18640.13763334152931023No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC18460.13630426419694563No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18280.13497518686458104No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17740.1309879548674873No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16910.12485943161269505No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16650.122939653243724No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT16530.12205360168881427No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16450.12146290065220779No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC16090.1188047459874786No Hit
GTCTCTGGTGGCTCCATCAGCAGGAGTACTTACTACTGGGGCTGGAGCCG15970.11791869443256889No Hit
GTTTATCACTGTCAGCAATATTATGGTATTCCGGGGTTCGGCCCTGGGAC15950.11777101917341729No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15820.11681112998893174No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15550.11481751399038487No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC15170.11201168406650408No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA14210.10492327162722631No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14070.10388954481316495No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG14010.1034465190357101No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13980.10322500614698266No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13960.10307733088783103No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13750.10152674066673903No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAA13580.10027150096395025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT8650.046.9609170
GTGGTAT5150.044.033711
TGGTATC5500.041.193492
CTAGACG350.001383955439.217414
GTATCAA29550.038.952551
GGTATCA12100.038.051171
TATCAAC32800.035.1651762
ATCAACG34300.033.3184853
TCAACGC34800.033.0333444
CAACGCA35300.032.377015
AACGCAG36100.031.663026
CTACGGA9450.031.05771369
TAGACGC450.00474173130.5080645
TGTTAGA10550.029.6666370
AGAGTAC39750.028.92830311
CGCAGAG40100.028.5912678
CAGAGTA40500.028.39259310
GCAGAGT40850.028.0663389
TCTACGG9550.027.351368
GCGGTCA9350.027.1947323
Produced by FastQC (version 0.11.5)