FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1045741
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT39850.3810695000004781No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18740.17920307227124116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11910.11389053312435869No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC11560.11054362409047747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG13000.050.11310270
TATACAC12800.043.528733
CTTATAC12850.042.8553961
TCGTATG10200.042.8490640
CGTATGC11500.041.77551341
CTCGTAT9400.040.8213739
TTATACA13900.039.842542
CCGTCTT10500.038.80010247
GTATCAA82100.038.7730521
AGTATCG350.001540224938.3720719
GTGGTAT11550.037.794291
ACGAGAC14600.036.58441522
ATCTCCG15300.036.19681510
CACGAGA15050.036.156721
TCTCGTA9750.035.33575438
GTATGCC14150.035.2957742
TACACAT15950.034.9321445
GCCGTCT11750.034.88764646
TATGCCG14250.034.41899543
CACATCT16000.034.3984957
Produced by FastQC (version 0.11.5)