FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1102079
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG143851.3052603307022455No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC17210.15615940418064403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT12400.048.217561
GTATCAA85850.037.0132941
TGGTATC16300.036.6625252
GGTATCA38400.035.601651
TATCAAC95800.032.977222
ATCAACG104600.030.1263453
TCAACGC104350.030.0993084
ATAAACG902.5419467E-729.8304633
CAACGCA106050.029.648455
AACGCAG108750.029.128356
CGTAGCA550.00649825828.60217968
ACGCAGA116800.027.1794787
CGCAGAG118250.026.7043068
GCAGAGT119600.026.4870519
AGAGTAC119850.026.431811
CAGAGTA120100.026.320910
TAGACCG851.741965E-423.6867475
GAACGGT901.8407713E-423.49458146
AGTACGG80500.023.46851713
GAGTACG82150.023.24328612
Produced by FastQC (version 0.11.5)