FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76230
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT11521.511216056670602No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGT3780.49586776859504134No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2150.28204119113210024No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC2040.2676111767020858No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC1990.26105207923389745No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1870.2453102453102453No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT1790.2348156893611439No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCGCGTATGCCGT1250.16397743670470943No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT1000.13118194936376756Illumina PCR Primer Index 7 (95% over 22bp)
CTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGCCTGT990.12987012987012986No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.1233110324019415No Hit
GTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC920.12068739341466615No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT900.11806375442739078No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCAGCTCGTATGCCGT890.11675193493375312No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGGCGTATGCCGT870.11412829594647776No Hit
CTGAAAGACAGGCTATTTTATTTCAAAAAAAGAAAAAAAAGTGGGCTCTGGGAACAGGGACTAGCCCGTACTCTG870.11412829594647776No Hit
GTATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGG850.1115046569592024No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.10756919847828939No Hit
GGTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC810.10625737898465172No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACCA100.007067512144.952370
CGTTTGC150.002200484570.1247341
CATCTAG150.002596157667.2634936
TCATCTA150.002653626766.89339435
GACGTCC201.10620684E-465.3649231
ATGGGGG702.2718268E-762.12241770
CTTATGC200.005239534756.3299251
TCTTATG200.005352646656.02707350
CGTGAAA701.8189894E-1255.1815557
GCGGACT200.00577151154.97155447
TATACAC3850.054.7035983
TTATACA3750.054.460472
CTTATAC3800.053.8145071
TTTGCCG200.00637973453.5982743
ATATCAA301.4247118E-553.253941
GTTTGCC200.00662444853.09115642
CCTCATG200.006876081752.59354841
AGGGGGG3750.052.18282770
TCTAGTA200.00761007651.26338
AAGTACG253.7270872E-451.1237871
Produced by FastQC (version 0.11.5)