FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397307
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT41591.0467975646036944No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9510.2393615013075531No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATGTCGTATGCCGT8680.2184708550314996No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC8540.21494713156324957No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7840.19732851422199962No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT6790.17090058821012466TruSeq Adapter, Index 3 (95% over 21bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5920.14900316380028542Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC4980.12534387765632118No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)