FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690968
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAATATGTTGATATTCCCCTCACTTTCGGCGGAGGGA30890.4470539880283892No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29900.4327262622871103No Hit
CAGTAATACACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCGCAGACCCACTGCCA28060.40609695383867267No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23690.34285234627363353No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGATGTATAAAAGACT20980.303632006113163No Hit
GTGTATTACTGTCAGCAATATGTTGATATTCCCCTCACTTTCGGCGGAGGGACCAGGGCGGAGATCAAACGAA20580.2978430260156766No Hit
GTAATACACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCGCAGACCCACTGCCA20520.29697467900105357No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19160.2772921466695998No Hit
CTTTTATACATCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA18420.2665825334892499No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18080.26166190040638637No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17920.2593463083673918No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17300.2503733892162879No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16710.2418346435724954No Hit
CTCCTAAGTTGCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGTGGGTCTG16080.23271699991895428No Hit
ATTCAGTGGCAGTGGGTCTGCGACAGATTTCACTCTCACCATCAGCAGCC16040.23213810190920564No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15780.22837526484583948No Hit
GTGCAGCCACAGTTCGTTTGATCTCCGCCCTGGTCCCTCCGCCGAAAGTG15430.22330990726053884No Hit
GTCCCTGACCGATTCAGTGGCAGTGGGTCTGCGACAGATTTCACTCTCAC15350.22215211124104153No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTTCCTGGGCATCTACCCGGGAA14970.21665258014842947No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14750.2134686410948119No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13740.19885146634865872No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13420.19422028227066956No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATA13310.19262831274386077No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13060.18901020018293177No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12900.18669460814393718No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11950.17294578041240694No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCGCCCTGGTCC11780.1704854638709752No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11490.16628845330029757No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC11450.1657095552905489No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGGAAATGAGCAACTTAGGAGGCTGTCCTGGTTTCTGCTGGTA11410.16513065728080026No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11140.16122309571499693No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCGCAGACCCACTGCCACTGAATCGGTCAGGGACC11040.15977585069062533No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11030.15963112618818817No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10890.1576049831540679No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGATG10440.1510923805443957No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10330.1495004110175869No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTGTATTACTGTCAGCAATATGTTGATATTCCCCTCACTTT10020.14501395144203494No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATACATCTCCAA9930.1437114309201005No Hit
GTATAAAAGACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA9850.14255363490060322No Hit
GTAATAGACGGCCGTGTCTGCGGCGCTCACAGACCTCAGTTTCAGGGACA9760.14125111437866877No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9740.14096166537379445No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9700.14038276736404579No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG9490.13734355281286542No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9410.13618575679336814No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATACATCTCCAACAATAAGAACTA9330.13502796077387086No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9080.13140984821294185No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8930.12923898067638445No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8840.12793646015444998No Hit
GACCAGGGCGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT8790.12721283764226418No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC8630.1248972456032696No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA8360.1209896840374663No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8340.12070023503259195No Hit
ATGTTGATATTCCCCTCACTTTCGGCGGAGGGACCAGGGCGGAGATCAAA8290.11997661252040616No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT8240.11925299000822036No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGGAAAT8180.11838464299359738No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGCTCATT8150.11795046948628592No Hit
GGCTGAAGATGTGGCAGTGTATTACTGTCAGCAATATGTTGATATTCCCC8110.11737157147653726No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8070.11679267346678861No Hit
ACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGTGGGTCTGCGACAGATTTCACTCTCACCATCAGCAGC8030.11621377545703998No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7990.11563487744729133No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7970.11534542844241702No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7960.11520070393997986No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7920.1146218059302312No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7850.11360873441317108No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7840.11346400991073392No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGCTCATTTCCTGGGCATCTACCCGGGAATC7740.11201676488636232No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7700.11143786687661367No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7560.10941172384249342No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG7360.10651723379375021No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG7310.10579361128156442No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC7160.10362274374500702No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7050.10203077421819823No Hit
GACCGATTCAGTGGCAGTGGGTCTGCGACAGATTTCACTCTCACCATCAG6960.1007282536962638No Hit
GCTGATGGTGAGAGTGAAATCTGTCGCAGACCCACTGCCACTGAATCGGT6940.10043880469138948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1900.052.4924241
CCTGCGT555.015893E-448.18572670
TAGCGAA3550.044.79236270
TGGTATC2500.041.2519872
GGTATCA5600.038.6905361
GTATCAA12550.037.5430071
CTTATAC1550.031.063371
ATCTTAT450.004693126330.5703011
CTAGCGA3700.029.88804869
ATCAACG15750.029.4657023
TCAACGC15700.029.3405844
CAACGCA15850.029.0629165
AACGCAG16300.028.2605636
CATGTCG500.00774213827.59317436
ACGAGAC1503.6379788E-1127.50530422
TATACAC1950.026.443583
TCTAGCG3750.026.26963668
GACCCTA1457.2759576E-1026.0901726
ACGGGAT1359.778887E-925.4641916
AGAGTAC18400.025.40883311
Produced by FastQC (version 0.11.5)