FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580243
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG126962.1880488002440357No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC22430.3865621817066298No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC14340.24713783707860326No Hit
GTATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGG9140.1575202113597234No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8580.14786908243615177No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8410.14493927544149607No Hit
GTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC8000.13787327033673824No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7150.12322423536345979No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6480.11167734897275797No Hit
GTATCAACGCAGAGTACGGGGATCTAAAAAAAAAAAAAAAAAAAAAAAAA6230.1073688092747349No Hit
TAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTT6120.10547305180760473No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6060.10443900228007921No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG5960.10271558640086997No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAAGT200.005403955.9474955
GTATTAT253.4409369E-452.016221
TATTATG253.448271E-451.9937932
TGTTATC253.448271E-451.9937932
ATAGTAC253.4512082E-451.9848311
AGTGTCC200.00914671648.9764725
TAAGTTG200.00932970748.731576
GCACTGT200.00932970748.731576
GTGGTAT12500.047.334761
TCATATG353.2352706E-546.4230272
ATCCATA307.435661E-444.49068532
GTGTTAT308.453384E-443.3468481
TAACGTT308.489356E-443.3094865
CTAACGT308.496565E-443.302024
TTACCCT308.496565E-443.302024
CTTGCTT700.001050622241.4651670
AGGAGTG407.132691E-540.6026425
CATATGC407.1399445E-540.5956423
TTTACTC407.1399445E-540.5956423
TTACTCG407.1399445E-540.5956424
Produced by FastQC (version 0.11.5)