FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009347
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28330.2806765165993459No Hit
GTATTACTGTCAGCAGTATGGGAACTTACCCTACACTTTTGGCCAGGGGA28020.27760522397153803No Hit
GTATGGGAACTTACCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT21420.21221641318595091No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA20850.20656919770901386No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA19150.18972662523393838No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18920.1874479242520164No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18780.18606088887171607No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18140.17972015570462885No Hit
GTCTGGGACAGACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATT17680.17516275374078488No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16660.1650572102557396No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16380.16228313949513895No Hit
CTCCTGCAGGGCCAGTCAGAGTCTTAGCAGAAGCAACTTAGCCTGGTACC15910.15762666357555924No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15570.15425814908054417No Hit
GTGTATTACTGTCAGCAGTATGGGAACTTACCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA14750.14613408471021364No Hit
GTTCCCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGT13660.13533502353501817No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13640.13513687562354673No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT13130.13008410388102407No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG13070.12948966014660965No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12700.12582392378438734No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC12570.12453596235982274No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11980.11869059897141418No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11390.11284523558300565No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC11190.11086375646829089No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11120.11017023877814072No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGTCAGTGGCCCTGCT10930.1082878336191617No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA10850.1074952419732758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15350.043.4618261
GTATCAA38300.040.135011
TCGTATG1900.036.39877740
TATCAAC43050.035.4546972
CGTATGC2100.032.9552241
ATCAACG46650.032.7923363
TCAACGC46850.032.7257234
GACGGAC2000.032.67565526
CAACGCA48000.031.9416685
ATCGTAT553.2256637E-431.41495539
AACGCAG48800.031.3475916
GCCGACT450.004672266530.59950331
ACGAGAC2250.030.5600822
ACGGACT2300.029.91351727
TGGTATC8350.029.6421722
GTGGTAT8600.029.5901991
CGCAGAG53050.028.6398
AGAGTAC53350.028.48077811
TGCCGTC2450.028.31086545
CGAGACG2450.028.06816123
Produced by FastQC (version 0.11.5)