FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009347
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25760.25521450997526124No Hit
GTATTACTGTCAGCAGTATGGGAACTTACCCTACACTTTTGGCCAGGGGA24920.24689229769345925No Hit
GTATGGGAACTTACCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT19250.19071736479129575No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA17930.17763960263417833No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17820.1765497891210852No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17500.1733794225375416No Hit
GTCTGGGACAGACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATT17240.1708034996884124No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16440.16287758322955337No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15200.15059241271832186No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14420.14286464417093428No Hit
CTCCTGCAGGGCCAGTCAGAGTCTTAGCAGAAGCAACTTAGCCTGGTACC14390.14256742230372704No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14200.14068501714474804No Hit
GTTCCCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGT13670.1354340974907539No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13590.13464150584486803No Hit
GTGTATTACTGTCAGCAGTATGGGAACTTACCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA13420.13295724859736047No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG13210.13087669552690998No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11900.1178980073255283No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11850.1174026375468496No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11540.11433134491904172No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGTCAGTGGCCCTGCT10950.10848598153063316No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC10520.10422580143399644No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA10370.10273969209796037No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10250.1015508046291315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15850.042.416271
GTATCAA39600.040.364211
TCGATTA601.5600669E-435.5507569
TATCAAC45900.034.733792
TCAACGC48450.032.9748274
ATCAACG48700.032.8039253
CAACGCA48850.032.633025
AACGCAG49500.032.4154856
GTGGTAT8350.031.6307811
TGGTATC8050.031.5172082
CTCGCTA1359.094947E-1230.50936923
GTACTAA450.004755146330.4896981
CGCAGAG52950.030.3681818
ATCGATT500.00491946730.2783468
AGAGTAC54600.029.4504611
ACGCAGA55300.028.9536727
GCAGAGT55850.028.7299299
CGGCGCG1155.5245694E-428.65466570
CAGAGTA57000.028.27059210
CGATTGT950.00752963527.74977970
Produced by FastQC (version 0.11.5)