FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1347780
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAAGGAGA49240.3653415245811631No Hit
GTCTCTGATGGCTCCATCAGCAGTAGTAGTCACTACTGGGGCTGGATCCG36570.2713350843609491No Hit
GTCTATTACTGTGCGAGGATGGACGCCCTAGGATATTGTAGTGGTGGGAG34520.25612488685096974No Hit
ATCCTAGGGCGTCCATCCTCGCACAGTAATAGACAGCCGTGTCTGCGGCG31420.23312410037246434No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT24640.18281915446141062No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC23140.1716897416492306No Hit
CCCTAGGATATTGTAGTGGTGGGAGCTGCTACCTGGAATACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG21690.16093130926412322No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20780.15417946549140066No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20390.15128581816023387No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19310.14327264093546424No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18870.14000801317722478No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18180.13488848328362196No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17350.12873020819421568No Hit
CTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGACTATTTATTATACTGGGAGCAC16920.12553977652139073No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTT16170.11997507011530073No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15370.11403938328213804No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA15130.11225867723218923No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14600.10832628470521895No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA14430.10706495125317188No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT13780.10224220570122722No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)