FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1347780
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAAGGAGA43310.32134324593034475No Hit
GTCTATTACTGTGCGAGGATGGACGCCCTAGGATATTGTAGTGGTGGGAG32230.23913398329104157No Hit
GTCTCTGATGGCTCCATCAGCAGTAGTAGTCACTACTGGGGCTGGATCCG31730.2354241790203149No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT27670.20530056834201427No Hit
ATCCTAGGGCGTCCATCCTCGCACAGTAATAGACAGCCGTGTCTGCGGCG27220.20196174449836024No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21060.15625695588300761No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20870.15484723026013147No Hit
CCCTAGGATATTGTAGTGGTGGGAGCTGCTACCTGGAATACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG19300.1431984448500497No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18080.1341465224294766No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17910.13288518897742954No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16880.1252429921797326No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTT16780.12450103132558726No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16620.12331389395895472No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15960.1184169523215955No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15880.11782338363827924No Hit
CTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGACTATTTATTATACTGGGAGCAC14560.10802950036356081No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14520.1077327160219027No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT14380.10669397082609922No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14350.10647138256985561No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)