FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences717373
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT34940.487054851520757No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC17440.24310923327195197No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14210.19808384201802967No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11650.16239808300563305No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8880.12378497657425076No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTAAA150.00276457266.2662629
TACACGT250.00595762754.58516369
AGCTAGT253.2709696E-452.5544621
TTAGATC253.278863E-452.5288163
CTTATAC6050.052.120131
GTGGTAT9650.050.376041
AGGGGGG7800.049.9453170
TATACAC6450.048.8640173
TACATAT452.2070944E-644.87211234
TTATACA7150.044.539292
GCTTGAA6900.044.1434156
GAGCGTA307.7725796E-444.0937328
ATACACA7150.044.0801244
CTTGAAA6950.043.96455457
TACACAT7200.043.774015
GACCTCT7100.042.70469726
GTATGCC7700.042.25056542
CCGTCTT7750.041.8103647
ATATCAA2050.041.6590271
TCGTATG7400.041.59663840
Produced by FastQC (version 0.11.5)