FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2370435
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36350.037.6262551
GTATCAA74300.036.6775931
CTTATAC10550.034.11611
TATCAAC88800.030.793332
TATACAC13450.030.5721283
GTGGTAT14750.030.2116071
ACGAGAC11200.029.98424322
TCAACGC92250.029.7164
ATCAACG92500.029.7097763
CAACGCA95100.028.933555
ATCTCCG12050.028.4355310
AACGCAG99050.027.8489046
CCCACGA12200.027.52245319
CGAGACT12450.027.25074623
TTATACA14600.026.2864482
ACGCAGA108750.025.3600947
CCACGAG13350.025.15160620
TCTCCGA14000.024.9654211
AGAGTAC110700.024.85619511
TCCGAGC14500.024.81350113
Produced by FastQC (version 0.11.5)