FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2370435
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91260.384992627935379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA78900.039.1596261
GGTATCA34250.036.3637431
TATCAAC92200.033.3090552
TCAACGC93400.032.7235874
ATCAACG94550.032.36313
CAACGCA95650.031.9963135
AACGCAG97100.031.69666
GTGGTAT15250.029.616371
ACGCAGA105300.029.2289147
CGCAGAG105950.029.0818758
AGAGTAC109050.028.22379511
CAGAGTA111500.027.634310
GCAGAGT113250.027.1468899
AGTACGG72100.025.65806413
GAGTACG73100.025.4490112
TGGTATC18700.023.957782
GTACGGG81750.022.20915414
TACGGGT15100.021.7448815
GAGTACT55700.021.67249512
TACGGGG32300.021.07236515
Produced by FastQC (version 0.11.5)