FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1365803
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACACCCAAGCACCGCTGCTGTCTGCTGATTGA28550.20903453865601407No Hit
CTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTCGAGTGGATGGGATGGATCAAACCTAACAGTGGTGA27880.2041289995702162No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26120.1912428073448367No Hit
CCCAAGCACCGCTGCTGTCTGCTGATTGACAGTGATAGTCAGCCTCGTCT23980.1755743690707957No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23510.17213317001060913No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC23030.168618753949142No Hit
GTAATACATGGCCGTGTCGTCAGATCTCAGGCCGCTCAACTCCATATAGACTGTGTTGATGGACGTGTCCCTGGT21260.15565934472248194No Hit
GTGTATATAGTTGCCGATGAAGGTGTATCCAGAAGCCTTGCAGGAGACCTTCACTGAGGCCCCAGGCTTCTTCAC21250.15558612772120137No Hit
GTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA21240.1555129107199208No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGC21120.15463430670455403No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCGAAG20580.1506805886354035No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT19910.1457750495496056No Hit
GTCTATATGGAGTTGAGCGGCCTGAGATCTGACGACACGGCCATGTATTACTGTGCGAGAGATGAGTCTGAGGTG19430.1422606334881385No Hit
GTGCTTGGGTGTTCGGCGGCGGGACCAAGTTGACCGTCCTGAGTCAGCCC19390.1419677654830162No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT18910.13845334942154908No Hit
CCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGTGTCTCATAAGT18670.1366961413908155No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG18630.13640327338569325No Hit
CACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18320.13413354634599572No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG17710.12966730926788125No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGT17710.12966730926788125No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACAC17570.12864227124995334No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCATCGG17420.12754401623074485No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC17170.1257135911987307No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC17120.12534750619232787No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCACGCGACCCCGAGAGCCT16830.1232242131551915No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16820.12315099615391092No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT16740.1225652601436664No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16660.12197952413342188No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATG16520.12095448611549396No Hit
ATGTATTACTGTGCGAGAGATGAGTCTGAGGTGCCTACGATTTTGTCCTACTTTGACTACTGGGGCCAGGGAACC16280.1191972780847604No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGC15990.11707398504762401No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15910.11648824903737949No Hit
GGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15570.11399887099384025No Hit
GTGATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA15450.11312026697847347No Hit
CTTATGAGACACACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGA15130.11077732293749537No Hit
GACTATCACTGTCAATCAGCAGACAGCAGCGGTGCTTGGGTGTTCGGCGGCGGGACCAAGTTGACCGTCCTGAGT15130.11077732293749537No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG14720.1077754258849922No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14290.1046270948299279No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13740.1006001597594968No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA13700.10030729175437453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3800.052.4326251
TGGTATC4350.045.7662962
GGTATCA11650.038.333161
GTATCAA26700.037.9549061
ATCAACG31200.032.337353
TCAACGC31800.031.61354
CAACGCA32550.031.0970385
AACGCAG33450.030.3618136
CGCAGAG37500.027.3602318
AGAGTAC38000.027.18083211
CAGAGTA38600.026.66943210
ACGCAGA38800.026.3550827
GCAGAGT39750.025.8115379
AGTACGG29300.024.35900713
GAGTACG29650.023.95574212
GTATTAT750.002043518722.9016061
GCTAGAC750.002055744622.8781223
GTAAGAC902.2045505E-422.878123
CCTTTGT11650.022.82007870
TTCGGGG11750.022.62586470
Produced by FastQC (version 0.11.5)