FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642925
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24060.37422716491037056No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTACAGCCTGAAGATG19470.30283470078158414No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17520.27250456896216513No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17040.2650386903604619No Hit
GTCCCAGACCCACTGCCACTGAACGTGGCTGGGATGCCAGTGGCCCTGTT15520.24139674145506862No Hit
GTATAACCCTTCCCTCAAGAGTCGACTCACCATCTCAGCAGACACGTCCAAGAATCAGTTCTCCCTGAGCTTGAG14910.23190885406540418No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACTA14550.22630944511412682No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14330.22288758408834622No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14250.22164327098806239No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13740.21371077497375277No Hit
GGGTTATACTGGGTGTGCCCATTGGGATAGATACGCCCAATCCACTCCAGTCCCTTCCCGGCGGACTGCCGGATC13620.21184430532332701No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13320.20717813119726253No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12920.20095656569584322No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11540.17949216471594664No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11430.17778123420305636No Hit
GACTTCACTCTCACCATCAGCAGCCTACAGCCTGAAGATGTTGCAGTTTACTTCTGCCAGCAGCGTTTCGAATGG11420.17762569506552087No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11040.17171520783917255No Hit
CCTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA10830.1684488859509274No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCCCGCCGAAGGTG10810.16813780767585645No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10600.16487148578761132No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10540.16393825096239842No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10520.16362717268732746No Hit
GTCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTTCTCATCTTTGATGCATCCAACAGGGCCACTGGCATC10450.16253839872457906No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10420.16207178131197264No Hit
GTCTCCAGCCACGCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCT10360.1611385464867597No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC10190.15849438114865652No Hit
GCCTACAGCCTGAAGATGTTGCAGTTTACTTCTGCCAGCAGCGTTTCGAATGGCCGCTCACCTTCGGCGGGGGGA9970.15507252012287592No Hit
TCTTTGATGCATCCAACAGGGCCACTGGCATCCCAGCCACGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT9850.15320605047245014No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9750.1516506590970953No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC9750.1516506590970953No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9420.1465178675584244No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9380.14589571100828247No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9210.14325154567017925No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGTAATTACTTAGTCTGGTACCAAC9110.14169615429482443No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9100.14154061515728897No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9060.14091845860714702No Hit
GTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGTAATTACTTAGTCTGGTA8960.1393630672317922No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8920.1387409106816503No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8740.13594120620601158No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8600.13376365828051484No Hit
GTCTCTGGCGACTCCATCCGTGATAGTGATTACTACTGGACCTGGATCCG8580.13345258000544388No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8580.13345258000544388No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8400.1306528755298052No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACGTGGCTGGGATGCCA8210.12769763191663103No Hit
TCCCAGCCACGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACC8110.1261422405412762No Hit
GCCTGAAGATGTTGCAGTTTACTTCTGCCAGCAGCGTTTCGAATGGCCGC7860.12225376210288914No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7750.12054283158999884No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTTCTCATCTTTGATGCATCCAACAGGGCCACTGGCATCCCAGCCACGTT7700.11976513590232142No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT7510.11680989228914726No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7500.11665435315161177No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7480.11634327487654081No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT7370.1146323443636505No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7370.1146323443636505No Hit
CTCCCAGGCTTCTCATCTTTGATGCATCCAACAGGGCCACTGGCATCCCA7350.11432126608857954No Hit
CACCATCAGCAGCCTACAGCCTGAAGATGTTGCAGTTTACTTCTGCCAGCAGCGTTTCGAATGGCCGCTCACCTT7280.11323249212583117No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC7110.11058832678772795No Hit
ATCCAACAGGGCCACTGGCATCCCAGCCACGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAG7080.11012170937512152No Hit
CAACAGGGCCACTGGCATCCCAGCCACGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG6990.10872185713730216No Hit
CACCTGCACTGTCTCTGGCGACTCCATCCGTGATAGTGATTACTACTGGA6920.1076330831745538No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA6900.10732200489948283No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6840.10638877007426994No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC6720.10452230042384415No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6610.10281136991095385No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6600.10265583077341835No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC6510.10125597853559902No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCCG200.003376325463.004269
TAGTTCG252.5992616E-455.0577057
TCTTATA453.0840965E-853.5366552
TCGGTCA200.00715359252.1192348
GTACAAT200.007412482551.6527561
CTTATAC1500.048.209241
TATACAC1650.043.802723
CGTATGC1700.040.7052141
CGAGACG1800.040.1587423
TTATACA1800.040.1524932
GGTATCA4350.039.5806541
CTCGTAT1850.039.2443439
AGACGGA1850.039.07640525
TCGTATG1900.038.2265340
GTATCAA9200.037.803831
ATGCCGT1850.037.4633844
TGCCGTC1900.036.49751345
GTATGCC2100.036.26403842
TACACAT2100.036.0552985
ACGAGAC2050.035.2613322
Produced by FastQC (version 0.11.5)