FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642925
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22060.3431193374032741No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTACAGCCTGAAGATG18390.28603647392775206No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15890.24715168954388148No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14470.22506513201384298No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14030.21822140996228173No Hit
GTATAACCCTTCCCTCAAGAGTCGACTCACCATCTCAGCAGACACGTCCAAGAATCAGTTCTCCCTGAGCTTGAG13960.2171326359995334No Hit
GTCCCAGACCCACTGCCACTGAACGTGGCTGGGATGCCAGTGGCCCTGTT13760.21402185324882372No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACTA13580.21122214877318507No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13480.20966675739783025No Hit
GGGTTATACTGGGTGTGCCCATTGGGATAGATACGCCCAATCCACTCCAGTCCCTTCCCGGCGGACTGCCGGATC13350.20764474860986895No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12710.1976902438075981No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12190.189602208655753No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11680.1816697126414434No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11540.17949216471594664No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11180.17389275576466928No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10960.17047089473888868No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10520.16362717268732746No Hit
GACTTCACTCTCACCATCAGCAGCCTACAGCCTGAAGATGTTGCAGTTTACTTCTGCCAGCAGCGTTTCGAATGG10350.16098300734922424No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10270.15973869424894038No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCCCGCCGAAGGTG9950.15476144184780496No Hit
GTCTCCAGCCACGCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCT9490.14760664152117275No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9480.14745110238363726No Hit
CCTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA9400.14620678928335343No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9300.1446513979079986No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC9280.14434031963292765No Hit
GTCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTTCTCATCTTTGATGCATCCAACAGGGCCACTGGCATC9080.14122953688221798No Hit
GTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGTAATTACTTAGTCTGGTA9000.13998522378193412No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGTAATTACTTAGTCTGGTACCAAC8930.13889644981918575No Hit
GCCTACAGCCTGAAGATGTTGCAGTTTACTTCTGCCAGCAGCGTTTCGAATGGCCGCTCACCTTCGGCGGGGGGA8930.13889644981918575No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8910.13858537154411477No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC8620.1340747365555858No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8480.13189718863008904No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8380.13034179725473422No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8370.13018625811719875No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8270.12863086674184393No Hit
TCTTTGATGCATCCAACAGGGCCACTGGCATCCCAGCCACGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT8260.12847532760430844No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7980.12412023175331494No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7970.12396469261577944No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7810.12147606641521172No Hit
GTCTCTGGCGACTCCATCCGTGATAGTGATTACTACTGGACCTGGATCCG7780.12100944900260528No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7560.11758758797682466No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7530.11712097056421822No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7490.1164988140140763No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG7410.11525450091379244No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT7390.11494342263872147No Hit
GCCTGAAGATGTTGCAGTTTACTTCTGCCAGCAGCGTTTCGAATGGCCGC7360.11447680522611503No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACGTGGCTGGGATGCCA7280.11323249212583117No Hit
TCCCAGCCACGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACC7270.11307695298829568No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7250.1127658747132247No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT7100.11043278765019249No Hit
CTCCCAGGCTTCTCATCTTTGATGCATCCAACAGGGCCACTGGCATCCCA6950.10809970058716024No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6560.10203367422327643No Hit
CACCTGCACTGTCTCTGGCGACTCCATCCGTGATAGTGATTACTACTGGA6480.10078936112299258No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6470.10063382198545708No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)