FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1299781
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23830.18333857780656895No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22720.17479867762338425No Hit
CTGTTATACCTTTGACAGTAATATGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC19890.15302577895814756No Hit
GTATAACAGTGCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT18380.1414084372675089No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17750.13656146689326895No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16640.12802156671008424No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16280.12525186935337568No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14510.11163419068289196No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14100.10847981313775167No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13520.10401752295194344No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13010.10009378502993965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7200.052.5786551
GTATCAA62250.038.533871
TATCAAC70550.034.029612
ATCAACG74400.032.31493
TCAACGC75050.031.9891914
CAACGCA76950.031.2887335
CGCGTAT553.3181004E-431.26480712
AACGCAG78200.030.9205446
GGTATCA37350.030.5912151
CGCAGAG83900.028.7345568
AGAGTAC85250.028.11815511
ACGCAGA86900.027.821727
CAGAGTA87000.027.6711510
GCAGAGT87950.027.4504649
AGTACGG57400.025.76350613
GAGTACG57600.025.61434412
ACGGGTA1901.8189894E-1225.34192316
CCGCGTA700.001358697124.56520511
GTACGGG64750.022.78588914
GAGTACT41700.022.0203212
Produced by FastQC (version 0.11.5)