FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391287
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT44360.3188414755546483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC8900.044.7224661
GTGGTAT11350.043.68731
TCGTATG8800.042.4949440
TCTCGTA8100.041.27918638
CGTATGC10100.038.8311841
CTCGTAT9000.038.38350739
GTATCAA81050.037.289691
ACGAGAC10750.036.708322
ATCTCGT8800.036.33530437
TATACAC11200.036.1186523
TTATACA11500.034.8857962
GCCGTCT10350.034.27506346
TACATCT9200.034.16137734
GGTATCA39800.033.9008561
GACCTCT11400.033.48845726
CCGTCTT10450.033.34603547
TATCAAC92400.033.275332
GAGACCT11850.033.03754424
TACACAT12850.032.2678685
GTATGCC12350.032.1328442
Produced by FastQC (version 0.11.5)