FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences747372
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACTTGTCATCTTTGGTAAAAACAATCGACCCTCTGGGATCCCA20050.26827336319797906No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG19360.2590410130430361No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA18640.24940725635961744No Hit
GCCTTGGGCTGACCTAGGACGGTCAGGTTGGTCCCTCCGCCGAAAAGCACGCGGTCACCACTACTGTCCCGGGAG17730.23723125832918546No Hit
GACCTAGGACGGTCAGGTTGGTCCCTCCGCCGAAAAGCACGCGGTCACCA17550.23482281915833078No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17170.22973833646430425No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC16910.22625947988418083No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15680.2098018122166739No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15560.20819618610277077No Hit
GTAGTGGTGACCGCGTGCTTTTCGGCGGAGGGACCAACCTGACCGTCCTA14810.1981610228908763No Hit
GTATAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAACTT14360.1921399249637396No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14290.19120330973062946No Hit
AGCCAGGACAGGCCCCTATACTTGTCATCTTTGGTAAAAACAATCGACCC14160.18946388144056775No Hit
CACCACTACTGTCCCGGGAGCAACAGAAATAGTCAGCCTCATCGTCCGCCTGAGCCCCAGTGATGGTCAAGGACG14150.18933007926440915No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT13330.17835830081940454No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT12220.1635062592658007No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGGTTGGTCCCTC11870.15882318310024995No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11450.15320349170158903No Hit
CTACTGTCCCGGGAGCAACAGAAATAGTCAGCCTCATCGTCCGCCTGAGC11150.14918942641683122No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10760.14397114154664611No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT10230.1368796262102407No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG10090.13500639574402037No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG10030.1342035826870688No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC10010.13393597833475165No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG9810.13125993481157977No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC9710.12992191304999384No Hit
GATCAGCACTGAGCACCGAGGATTCCCCATGGAACTGGGGCTCCGCTGGG9410.12590784776523603No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC9270.12403461729901576No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGTTGTGTCTGTGGCCTTGGGA9050.12109096942352669No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9020.12068956289505092No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA8870.11868253025267203No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC8760.1172107063149275No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT8740.11694310196261032No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC8460.11319664103016973No Hit
GTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAACTTCTGAGGCTGTCCCCTTGGCATGTGATCCTGACTGT8450.11306283885401111No Hit
GGACAGTAGTGGTGACCGCGTGCTTTTCGGCGGAGGGACCAACCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC8400.11239382797321815No Hit
ACCTAGGACGGTCAGGTTGGTCCCTCCGCCGAAAAGCACGCGGTCACCAC8390.11226002579705957No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC8310.11118960838779081No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT8230.11011919097852207No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT8070.10797835615998458No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7930.10610512569376428No Hit
GAAATAAACAGCCGAGTCCTCGGCTCTCAGGCTGTTCATTTGTAGATACA7860.10516851046065412No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAATTCATGCCATAGGAAT7830.10476710393217835No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCACGGCG7760.10383048869906819No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGTT7750.10369668652290961No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA7710.10316147781827523No Hit
CCACTACTGTCCCGGGAGCAACAGAAATAGTCAGCCTCATCGTCCGCCTGAGCCCCAGTGATGGTCAAGGACG7670.10262626911364088No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7650.10235866476132369No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)