FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences747372
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG21920.29329437013963594No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA19130.25596356299138845No Hit
CCCCTATACTTGTCATCTTTGGTAAAAACAATCGACCCTCTGGGATCCCA19020.254491739053644No Hit
GCCTTGGGCTGACCTAGGACGGTCAGGTTGGTCCCTCCGCCGAAAAGCACGCGGTCACCACTACTGTCCCGGGAG16230.21716093190539654No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15560.20819618610277077No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC14570.19494977066307007No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14260.19080190320215368No Hit
GACCTAGGACGGTCAGGTTGGTCCCTCCGCCGAAAAGCACGCGGTCACCA14050.18799205750282322No Hit
CACCACTACTGTCCCGGGAGCAACAGAAATAGTCAGCCTCATCGTCCGCCTGAGCCCCAGTGATGGTCAAGGACG14000.18732304662203025No Hit
GTATAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAACTT13260.17742168558629437No Hit
AGCCAGGACAGGCCCCTATACTTGTCATCTTTGGTAAAAACAATCGACCC13220.17688647688166No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG13210.1767526747055014No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12900.17260480724458502No Hit
GTAGTGGTGACCGCGTGCTTTTCGGCGGAGGGACCAACCTGACCGTCCTA12840.17180199418763345No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11920.15949219398104292No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT11690.15641474392939528No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11230.1502598438261No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC10140.13567540662481334No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGGTTGGTCCCTC10040.1343373848632274No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC10020.13406978051091023No Hit
CTACTGTCCCGGGAGCAACAGAAATAGTCAGCCTCATCGTCCGCCTGAGC9880.13219655004468991No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC9640.1289852978168837No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG9410.12590784776523603No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG9140.12229518900895404No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC9100.12175998030431966No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8670.11600648672950016No Hit
GATCAGCACTGAGCACCGAGGATTCCCCATGGAACTGGGGCTCCGCTGGG8650.115738882377183No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGTTGTGTCTGTGGCCTTGGGA8590.11493606932023143No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT8130.10878116921693615No Hit
GTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAACTTCTGAGGCTGTCCCCTTGGCATGTGATCCTGACTGT7960.10650653222224007No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT7950.10637273004608147No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAATTCATGCCATAGGAAT7920.10597132351760569No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT7900.1057037191652885No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT7740.10356288434675101No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA7560.10115444517589635No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC7550.10102064299973773No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT7540.10088684082357915No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA7520.10061923647126196No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGTT7490.10021782994278619No Hit
GGACAGTAGTGGTGACCGCGTGCTTTTCGGCGGAGGGACCAACCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC7490.10021782994278619No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGTA150.002376717768.8420911
TCGACTT350.00432861959.16989570
AACTAAG200.00733499551.7910437
AGTCGAA200.007356440651.75281532
GATTACG200.00740143251.6730731
AATACGT200.00742302251.6350210
GTGGTAT1900.047.140351
TGGTATC2050.043.655962
GTATCAA13900.042.6271861
CTAGACA350.001365919639.3199124
TGGTCGT450.001702492237.59507469
TATCAAC15600.037.5101132
GGTATCA5450.037.2931061
GGATGGG10150.034.68580270
GTATCAC400.002614758734.4487151
ATCAACG17000.034.2071233
TCAACGC17100.033.801334
CAACGCA17900.032.6838155
AACGCAG18250.032.0612956
TCCGAGA3500.031.55728170
Produced by FastQC (version 0.11.5)